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Species: Mus musculus
Genetic Insert: nE-mHP1alpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Comments: PCR on gene block containing mutations (four serine residues in the N-terminus converted to glutamic acid) switched out HP1 using BspEI (Kpn2I) and BamHI.
For additional reference on HP1alpha, please see Larson et al. 2017 Nature, PMID 28636604, https://doi.org/10.1038/nature22822
Proper citation: RRID:Addgene_181895 Copy
Species: E. coli
Genetic Insert: ribA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5021; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35863619
Comments: Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels.
Proper citation: RRID:Addgene_188578 Copy
Species: E. coli
Genetic Insert: ribA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5021; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35863619
Comments: Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels.
Proper citation: RRID:Addgene_188579 Copy
Species: Synthetic
Genetic Insert: his6-HA3-SNAPf
Vector Backbone Description: Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188941 Copy
Species: Synthetic
Genetic Insert: his6-HA3-CLIP
Vector Backbone Description: Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188942 Copy
Species: Synthetic
Genetic Insert: his6-HA3-HALO
Vector Backbone Description: Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188943 Copy
Species: Homo sapiens
Genetic Insert: NT473 ITGA5 point mutation
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2626; Vector Backbone:pUC-GW-Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35091451
Proper citation: RRID:Addgene_173811 Copy
Species: Homo sapiens
Genetic Insert: NT1745 ITGA5 point mutation
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2626; Vector Backbone:pUC-GW-Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35091451
Proper citation: RRID:Addgene_173812 Copy
Species: Homo sapiens
Genetic Insert: integrin b3
Vector Backbone Description: Backbone Marker:Atum; Vector Backbone:pD2529CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36113427
Proper citation: RRID:Addgene_189797 Copy
Species: Homo sapiens
Genetic Insert: Keratin-emiRFP2
Vector Backbone Description: Backbone Size:3933; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34913537
Comments: Please note: Plasmid contains a V282L mutation in Keratin. This mutation did not affect imaging by the depositing lab.
Proper citation: RRID:Addgene_178972 Copy
Species: Homo sapiens
Genetic Insert: HUWE1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34314700
Proper citation: RRID:Addgene_187147 Copy
Species: Homo sapiens
Genetic Insert: hQPRTase
Vector Backbone Description: Backbone Marker:Liu, H & Naismith, JH, University of St Andrews; Backbone Size:5364; Vector Backbone:pEHISTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17868694
Proper citation: RRID:Addgene_92411 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28495799
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.
Proper citation: RRID:Addgene_92370 Copy
Species: Homo sapiens
Genetic Insert: hQPRTase
Vector Backbone Description: Backbone Marker:Liu, H & Naismith, JH, University of St Andrews; Backbone Size:5364; Vector Backbone:pEHISTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17868694
Comments: Addgene's sequencing results found an A39T mutation in HQPRT. This mutation is not known to affect enzyme function.
Proper citation: RRID:Addgene_92407 Copy
Species: Homo sapiens
Genetic Insert: hQPRTase
Vector Backbone Description: Backbone Marker:Liu, H & Naismith, JH, University of St Andrews; Backbone Size:5364; Vector Backbone:pEHISTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17868694
Proper citation: RRID:Addgene_92403 Copy
Genetic Insert: 5HT6-YFP-Inpp5e
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:eYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28086093
Proper citation: RRID:Addgene_96808 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:Addgene #47320; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28656978
Proper citation: RRID:Addgene_96879 Copy
Vector Backbone Description: Backbone Size:10620; Vector Backbone:p15a ori vector; Vector Types:Mammalian Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35086530
Comments: This plasmid is for ES cell targeting of an intersectional Cre/FLP responsive cassette into the Rosa26 locus. It consists of a 5' homology arm, CAG promoter, FRT-flanked neomycin resistance and stop cassette, LoxP-flanked EcoRV cloning site and stop cassette, PmeI cloning site, WPRE and bgh pA elements, and a 3' homology arm. A subtractive cDNA of interest (expressed in response to FLP but not Cre recombination) can be cloned into the EcoRV site. The intersectional cDNA of interest (expressed in response to both Cre and FLP recombination) can be cloned into the PmeI site. This vector lacks the sgRNA target sequence found in the px330_Rosa_sgRNA plasmid.
Proper citation: RRID:Addgene_97012 Copy
Species: Arabidopsis thaliana
Genetic Insert: SEIPIN1
Vector Backbone Description: Backbone Size:11752; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26362606
Comments: The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
Proper citation: RRID:Addgene_96973 Copy
Species: Arabidopsis thaliana
Genetic Insert: SEIPIN2
Vector Backbone Description: Backbone Size:12460; Vector Backbone:pMDC43; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26362606
Comments: The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
Proper citation: RRID:Addgene_96977 Copy
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