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  • Bacterial Resistance:kanamycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGFP-nE-mHP1alpha
 
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RRID:Addgene_181895 nE-mHP1alpha Mus musculus Kanamycin PMID:32101700 PCR on gene block containing mutations (four serine residues in the N-terminus converted to glutamic acid) switched out HP1 using BspEI (Kpn2I) and BamHI. For additional reference on HP1alpha, please see Larson et al. 2017 Nature, PMID 28636604, https://doi.org/10.1038/nature22822 Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin S11E, S12E, S13E, S14E 2026-08-15 01:23:42 0
pDL52 (clone45)
 
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RRID:Addgene_188578 ribA E. coli Kanamycin PMID:35863619 Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels. Backbone Marker:Novagen; Backbone Size:5021; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimization, synthetic ribosome binding sites 2026-08-15 01:23:43 0
pDL52 (clone76)
 
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RRID:Addgene_188579 ribA E. coli Kanamycin PMID:35863619 Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels. Backbone Marker:Novagen; Backbone Size:5021; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimization, synthetic ribosome binding sites 2026-08-15 01:23:41 0
pET28-his6-HA3-SNAPf
 
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RRID:Addgene_188941 his6-HA3-SNAPf Synthetic Kanamycin PMID:35944214 Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:42 0
pET28-his6-HA3-CLIP
 
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RRID:Addgene_188942 his6-HA3-CLIP Synthetic Kanamycin PMID:35944214 Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:44 0
pET28-his6-HA3-HALO
 
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RRID:Addgene_188943 his6-HA3-HALO Synthetic Kanamycin PMID:35944214 Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:42 0
pITGA5_473G>A
 
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RRID:Addgene_173811 NT473 ITGA5 point mutation Homo sapiens Kanamycin PMID:35091451 Backbone Marker:Genewiz; Backbone Size:2626; Vector Backbone:pUC-GW-Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:44 0
pITGA5_1745G>U
 
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RRID:Addgene_173812 NT1745 ITGA5 point mutation Homo sapiens Kanamycin PMID:35091451 Backbone Marker:Genewiz; Backbone Size:2626; Vector Backbone:pUC-GW-Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:42 0
pD2529-CAG-b3
 
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RRID:Addgene_189797 integrin b3 Homo sapiens Kanamycin PMID:36113427 Backbone Marker:Atum; Vector Backbone:pD2529CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:42 0
pKeratin-emiRFP2-N1
 
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RRID:Addgene_178972 Keratin-emiRFP2 Homo sapiens Kanamycin PMID:34913537 Please note: Plasmid contains a V282L mutation in Keratin. This mutation did not affect imaging by the depositing lab. Backbone Size:3933; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:44 0
pDONR221-HUWE1_UIM-mut
 
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RRID:Addgene_187147 HUWE1 Homo sapiens Kanamycin PMID:34314700 Vector Backbone:pDONR221; Vector Types:Entry clone; Bacterial Resistance:Kanamycin A1376G/S1382A 2026-08-15 01:23:42 0
pEHIShqprtR138Q
 
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RRID:Addgene_92411 hQPRTase Homo sapiens Kanamycin PMID:17868694 Backbone Marker:Liu, H & Naismith, JH, University of St Andrews; Backbone Size:5364; Vector Backbone:pEHISTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin R138Q 2026-08-15 01:22:30 0
pCMV-Tag/SP S245X with 5'UTR
 
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RRID:Addgene_92370 spastin Homo sapiens Kanamycin PMID:28495799 Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody. Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin S245X 2026-08-15 01:22:29 0
pEHIShqprtR102Q
 
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RRID:Addgene_92407 hQPRTase Homo sapiens Kanamycin PMID:17868694 Addgene's sequencing results found an A39T mutation in HQPRT. This mutation is not known to affect enzyme function. Backbone Marker:Liu, H & Naismith, JH, University of St Andrews; Backbone Size:5364; Vector Backbone:pEHISTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin R102Q 2026-08-15 01:22:30 0
pEHIShqprtK139A
 
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RRID:Addgene_92403 hQPRTase Homo sapiens Kanamycin PMID:17868694 Backbone Marker:Liu, H & Naismith, JH, University of St Andrews; Backbone Size:5364; Vector Backbone:pEHISTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin K139A 2026-08-15 01:22:30 0
5HT6-YFP-Inpp5e
 
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1+ mentions
RRID:Addgene_96808 5HT6-YFP-Inpp5e Kanamycin PMID:28086093 Backbone Marker:Clontech; Vector Backbone:eYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:22:30 2
pWT032a
 
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RRID:Addgene_96879 mCherry Other Kanamycin PMID:28656978 Vector Backbone:Addgene #47320; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:22:30 0
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA
 
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RRID:Addgene_97012 Kanamycin PMID:35086530 This plasmid is for ES cell targeting of an intersectional Cre/FLP responsive cassette into the Rosa26 locus. It consists of a 5' homology arm, CAG promoter, FRT-flanked neomycin resistance and stop cassette, LoxP-flanked EcoRV cloning site and stop cassette, PmeI cloning site, WPRE and bgh pA elements, and a 3' homology arm. A subtractive cDNA of interest (expressed in response to FLP but not Cre recombination) can be cloned into the EcoRV site. The intersectional cDNA of interest (expressed in response to both Cre and FLP recombination) can be cloned into the PmeI site. This vector lacks the sgRNA target sequence found in the px330_Rosa_sgRNA plasmid. Backbone Size:10620; Vector Backbone:p15a ori vector; Vector Types:Mammalian Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:22:32 0
pMDC32-SEIPIN1
 
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RRID:Addgene_96973 SEIPIN1 Arabidopsis thaliana Kanamycin PMID:26362606 The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html Backbone Size:11752; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:22:32 0
pMDC43-SEIPIN2
 
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RRID:Addgene_96977 SEIPIN2 Arabidopsis thaliana Kanamycin PMID:26362606 The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html Backbone Size:12460; Vector Backbone:pMDC43; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:22:32 0

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