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Species: Homo sapiens
Genetic Insert: BRSK2
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001256627.1,NM_001256629.1,NM_001256630.1,NM_001282218.1,NM_003957.3. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_78025 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Addgene plasmid 52963; Backbone Size:10183; Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27329820
Proper citation: RRID:Addgene_78164 Copy
Species: Homo sapiens
Genetic Insert: ESR1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pmiRGlo; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25333260
Proper citation: RRID:Addgene_78134 Copy
Species: Homo sapiens
Genetic Insert: miR373
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25333260
Proper citation: RRID:Addgene_78127 Copy
Species: Homo sapiens
Genetic Insert: miR155
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25333260
Proper citation: RRID:Addgene_78126 Copy
Species: Homo sapiens
Genetic Insert: miR766
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25333260
Proper citation: RRID:Addgene_78124 Copy
Species: Homo sapiens
Genetic Insert: MBD2b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17634428
Proper citation: RRID:Addgene_78142 Copy
Species: Homo sapiens
Genetic Insert: PAX3
Vector Backbone Description: Backbone Marker:Stephen Elledge; Backbone Size:11791; Vector Backbone:pInducer20 (Addgene plasmid #44012); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please contact Dr. Alexander if you have any questions.
Proper citation: RRID:Addgene_78336 Copy
Species: Homo sapiens
Genetic Insert: MYOD1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag2b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please contact Dr. Alexander if you have any questions.
Proper citation: RRID:Addgene_78329 Copy
Species: Homo sapiens
Genetic Insert: SRF
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag2b; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please contact Dr. Alexander if you have any questions.
Proper citation: RRID:Addgene_78343 Copy
Species: Homo sapiens
Genetic Insert: MYOG
Vector Backbone Description: Backbone Marker:Stephen Elledge; Backbone Size:11791; Vector Backbone:pInducer20 (Addgene plasmid #44012); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please contact Dr. Alexander if you have any questions.
Proper citation: RRID:Addgene_78342 Copy
Species: Homo sapiens
Genetic Insert: SRF
Vector Backbone Description: Backbone Marker:Stephen Elledge; Backbone Size:11791; Vector Backbone:pInducer20 (Addgene plasmid #44012); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please contact Dr. Alexander if you have any questions.
Proper citation: RRID:Addgene_78345 Copy
Species: Homo sapiens
Genetic Insert: SRF
Vector Backbone Description: Backbone Marker:Stephen Elledge; Backbone Size:11791; Vector Backbone:pInducer20 (Addgene plasmid #44012); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: hSRF contains a T505N mutation compared to NP_003122.1. The depositor states that this mutation should not affect plasmid function. Please contact Dr. Alexander if you have any questions.
Proper citation: RRID:Addgene_78344 Copy
Species: Homo sapiens
Genetic Insert: RAB5A
Vector Backbone Description: Backbone Marker:Goodrum Lab, Addgene plasmid ; Vector Backbone:pCIG3; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Subcloned from mCherry-Rab5CA(Q79L) from Sergio Grinstein (Addgene plasmid # 35138)
Proper citation: RRID:Addgene_78268 Copy
Species: Homo sapiens
Genetic Insert: NDUFS2 K62A
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:5500; Vector Backbone:pcDNA3.1/myc-His(-)A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34852219
Proper citation: RRID:Addgene_127501 Copy
Species: Homo sapiens
Genetic Insert: EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
Vector Backbone Description: Vector Backbone:pEF-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The EGFP coding sequence in a reverse complement orientation flanked by attB and attP integrase 4 attachment sites were cloned into pEF-GFP plasmid (Addgene #11154) replacing the original EGFP forward coding sequence. The attB/P integrase 2 attachment sites sequences were obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127505 Copy
Species: Homo sapiens
Genetic Insert: MCPIP1
Vector Backbone Description: Backbone Size:4714; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21115689
Proper citation: RRID:Addgene_127459 Copy
Species: Homo sapiens
Genetic Insert: 5S rRNA with F30 folding scaffold
Vector Backbone Description: Backbone Size:8205; Vector Backbone:pSLQ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29440634
Proper citation: RRID:Addgene_127582 Copy
Species: Homo sapiens
Genetic Insert: mgU2-47 scaRNA with Mango II tag
Vector Backbone Description: Backbone Size:8205; Vector Backbone:pSLQ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29440634
Proper citation: RRID:Addgene_127586 Copy
Species: Homo sapiens
Genetic Insert: Miro2
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29311115
Proper citation: RRID:Addgene_127614 Copy
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