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pMid-TCGC-mNeongreen
 
Resource Report
Resource Website 		RRID:Addgene_241171 mNeongreen Synthetic Spectinomycin PMID:41630603 Vector Backbone:pU6a:sgRNA#23; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin TCGC linker 2026-08-29 01:24:54 0
linker-P2A-lifeact-mNeongreen-ORF-2
 
Resource Report
Resource Website 		RRID:Addgene_241173 mNeongreen Synthetic Spectinomycin PMID:41630603 Vector Backbone:pU6a:sgRNA#25; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin TCGC linker 2026-08-29 01:24:55 0
linker-P2A-mNeongreen-ORF1
 
Resource Report
Resource Website 		RRID:Addgene_241176 mNeongreen Synthetic Spectinomycin PMID:41630603 Vector Backbone:pU6a:sgRNA#28; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin TCGC linker 2026-08-29 01:24:55 0
linker-P2A-mNeongreen-ORF3
 
Resource Report
Resource Website 		RRID:Addgene_241178 mNeongreen Synthetic Spectinomycin PMID:41630603 Vector Backbone:pU6a:sgRNA#30; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin TCGC linker 2026-08-29 01:24:53 0
linker-P2A-mNeongreen-ORF2
 
Resource Report
Resource Website 		RRID:Addgene_241177 mNeongreen Synthetic Spectinomycin PMID:41630603 Vector Backbone:pU6a:sgRNA#29; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin TCGC linker 2026-08-29 01:24:54 0
pCR178
 
Resource Report
Resource Website 		RRID:Addgene_240641 mScarlet-I Synthetic Ampicillin Backbone Marker:Baker et al., 1999; Backbone Size:4108; Vector Backbone:pCS107; Vector Types:Vertebrate gene expression and general in vitro transcription; Bacterial Resistance:Ampicillin 2026-08-29 01:24:52 0
ISA894: vioABCDE phagemid
 
Resource Report
Resource Website 		RRID:Addgene_241972 vioABCDE Chloramphenicol PMID:35150576 Vector Backbone:p15a, CamR, coi(Y35H) phagemid; Vector Types:Bacterial Expression, Synthetic Biology, phagemid; Bacterial Resistance:Chloramphenicol 2026-08-29 01:24:55 0
pCR175
 
Resource Report
Resource Website 		RRID:Addgene_240640 mTurquoise2 Synthetic Ampicillin Backbone Marker:Baker et al., 1999; Backbone Size:4108; Vector Backbone:pCS107; Vector Types:Vertebrate gene expression and general in vitro transcription.; Bacterial Resistance:Ampicillin 2026-08-29 01:24:53 0
FRT-acrymCherry-FRT
 
Resource Report
Resource Website 		RRID:Addgene_241179 acry-mCherry Synthetic Spectinomycin PMID:41630603 Vector Backbone:pU6a:sgRNA#31; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin TCGC linker 2026-08-29 01:24:55 0
pCR197
 
Resource Report
Resource Website 		RRID:Addgene_240643 AIPGENE865 promoter Exaiptasia diaphana Ampicillin The whole intergenic region between AIPGENE865 (KXJ12416.1; Elongation Factor 1alpha) and AIPGENE899 was cloned as promoter fragment. The original gene annotation for AIPGENE865 is incorrect, the automatic NCBI annotation is correct in regards of exons, introns and translation start site. We found that Aiptasia strain CC7 was actually heterozygous for that locus: one allele was mostly in agreement with the genome sources but the other one had a couple of SNPs and a large, 749-bp insert in the intergenic region. The longer version with insert was used as promoter for this plasmid. The Aiptasia MEK2 locus was found to be erroneously split into AIPGENES3280 & 23794 on two different scaffolds due to an assembly gap in the Baumgarten et al., 2015 genome. Correct CDS was identified from sequencing based on cDNA of strain CC7. AmNeonGreen is a version of mNeonGreen codon-optimized for Aiptasia (based on the transcriptome of Lehnert et al., 2014) with the identical protein sequence. Baumgarten S, Simakov O, Esherick LY, Liew YJ, Lehnert EM, Michell CT, Li Y, Hambleton EA, Guse A, Oates ME, et al. 2015. The genome of Aiptasia, a sea anemone model for coral symbiosis. Proc Natl Acad Sci. 112(38):11893–11898. doi:10.1073/pnas.1513318112. Lehnert EM, Mouchka ME, Burriesci MS, Gallo ND, Schwarz JA, Pringle JR. 2014. Extensive differences in gene expression between symbiotic and aposymbiotic cnidarians. G3 Bethesda Md. 4(2):277–295. doi:10.1534/g3.113.009084. Backbone Marker:Tim Howes; Backbone Size:3854; Vector Backbone:pT2HE; Vector Types:Gene expression and genomic integration in fish; Bacterial Resistance:Ampicillin naturally occurring 749-bp insertion at bp 628 (from bp 1 of plasmid map), corrected exon/intron positions (in agreement with XM_021048156.2). 2026-08-29 01:24:54 0
pCR241
 
Resource Report
Resource Website 		RRID:Addgene_240644 AIPGENE865 promoter Exaiptasia diaphana Ampicillin The whole intergenic region between AIPGENE865 (KXJ12416.1; Elongation Factor 1alpha) and AIPGENE899 was cloned as promoter fragment. The original gene annotation for AIPGENE865 is incorrect, the automatic NCBI annotation is correct in regards of exons, introns and translation start site. We found that Aiptasia strain CC7 was actually heterozygous for that locus: one allele was mostly in agreement with the genome sources but the other one had a couple of SNPs and a large, 749-bp insert in the intergenic region. The longer version with insert was used as promoter for this plasmid. AmNeonGreen is a version of mNeonGreen codon-optimized for Aiptasia (based on the transcriptome of Lehnert et al., 2014) with the identical protein sequence. Lehnert EM, Mouchka ME, Burriesci MS, Gallo ND, Schwarz JA, Pringle JR. 2014. Extensive differences in gene expression between symbiotic and aposymbiotic cnidarians. G3 Bethesda Md. 4(2):277–295. doi:10.1534/g3.113.009084. Backbone Marker:Tim Howes; Backbone Size:3366; Vector Backbone:pT2HE; Vector Types:Gene expression and genomic integration in fish; Bacterial Resistance:Ampicillin naturally occurring 749-bp insertion at bp 628 (from bp 1 of plasmid map), corrected exon/intron positions (in agreement with XM_021048156.2). 2026-08-29 01:24:52 0
pREC1010lac-Eco2
 
Resource Report
Resource Website 		RRID:Addgene_240692 Eco2 RT, Eco2 ncRNA, CspRecT and EcSSB Synthetic Kanamycin PMID:42026131 Please visit https://doi.org/10.1101/2025.06.16.660010 for bioRxiv preprint. Backbone Marker:George Church (Addgene #138474); Vector Backbone:pORTMAGE-Ec1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin WT 2026-08-29 01:24:54 0
Ec dnaQ-pCDFDuet
 
Resource Report
Resource Website 		RRID:Addgene_241263 dnaQ E. coli Streptomycin Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-29 01:24:54 0
Ec FLAG-S6-dnaB-pET
 
Resource Report
Resource Website 		RRID:Addgene_241268 FLAG-S6-dnaB E. coli Ampicillin Vector Backbone:pET(3c); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:24:55 0
Ec FLAG-dnaB-pET
 
Resource Report
Resource Website 		RRID:Addgene_241267 FLAG-dnaB E. coli Ampicillin Vector Backbone:pET(3c); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:24:55 0
pREC1010lac-Csp1
 
Resource Report
Resource Website 		RRID:Addgene_240697 Csp1 RT, Csp1 ncRNA, CspRecT and EcSSB Synthetic Kanamycin PMID:42026131 Please visit https://doi.org/10.1101/2025.06.16.660010 for bioRxiv preprint. Backbone Marker:George Church (Addgene #138474); Vector Backbone:pORTMAGE-Ec1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin WT 2026-08-29 01:24:53 0
Ec dnaC-pET(11a)
 
Resource Report
Resource Website 		RRID:Addgene_241269 dnaC E. coli Ampicillin Vector Backbone:pET(11a); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:24:54 0
Sc FLAG-rad24-pRSFDuet
 
Resource Report
Resource Website 		RRID:Addgene_240733 FLAG-rad24 Saccharomyces cerevisiae Kanamycin Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:24:54 0
Sc [rad24 + RFC5]-pLANT/RIL
 
Resource Report
Resource Website 		RRID:Addgene_240732 Rad24 Saccharomyces cerevisiae Kanamycin Vector Backbone:pLANT/RIL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:24:54 0
Sc FLAG-S6-rad24- pRS404/GAL-L
 
Resource Report
Resource Website 		RRID:Addgene_240736 FLAG-S6-rad24 Saccharomyces cerevisiae Ampicillin Vector Backbone:pRS404/GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:24:54 0

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