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Species: Mus musculus
Genetic Insert: sgSik3_1st/sgNT1
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177214 Copy
Species: Mus musculus
Genetic Insert: sgSik1_1st/sgSik3_2nd
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177217 Copy
Species: Homo sapiens
Genetic Insert: YTHDC1 (YTH domain)
Vector Backbone Description: Vector Backbone:Addgene 101221; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34375583
Proper citation: RRID:Addgene_177126 Copy
Species: Homo sapiens
Genetic Insert: YTHDC1
Vector Backbone Description: Vector Backbone:modified from Addgene 61425; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34375583
Proper citation: RRID:Addgene_177129 Copy
Species: Homo sapiens
Genetic Insert: dCasRx-FTO
Vector Backbone Description: Vector Backbone:Addgene 61425; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34375583
Proper citation: RRID:Addgene_177120 Copy
Species: Mus musculus
Genetic Insert: sgNeo1/sgPrkaa1/sgPrkaa2
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177233 Copy
Species: Mus musculus
Genetic Insert: sgMark2/sgMark3/sgMark4
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177235 Copy
Species: Mus musculus
Genetic Insert: sgNeo1/sgNuak1/sgNuak2
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177234 Copy
Species: Mus musculus
Genetic Insert: sgNeo1/sgNT1/sgSnrk
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177236 Copy
Genetic Insert: MG1655 PlacZYA::Plac-gfp-muNS-kan
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24361104
Comments: GFP-muNS expression is under the control of Plac promoter integrated into the endogenous lac locus. The native lac operon (lacZYA) is deleted. Kanamycin resistance casette is inserted downstream of the gfp-muNS gene for cloning purposes.
Cloning method: Lambda RED recombination. The gfp-μNS fragment was amplified from plasmid pER12 (pBAD322A-gfp-μNS), which encodes an N-terminal GFP fusion to residues 471-721 of μNS (Broering et al., 2005) and then digested by KpnI/XhoI. The nptI gene was amplified from pKD4 plasmid and digested by XhoI/SmaI. Then, gfp-μNS and nptI gene fragments were triple ligated into the pKS plasmid. The resulting plasmid was used as a PCR template to generate a gfp-μNS-nptI fragment with flanking regions complementary to lacI and cynX, respectively. This fragment was then used to replace the lac operon (lacZYA) by one-step λ-recombination (Datsenko and Wanner, 2000). The resulting gfp-μNS-nptI replacement of lacZYA was transduced back into MG1655 by P1-phage to generate strain CJW4617. See Parry et al., 2014 for more details. This strain doesn't contain any plasmid.
Proper citation: RRID:Addgene_177118 Copy
Species: E. coli
Genetic Insert: sgNeo1/sgNT1/sgNeo2
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177231 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: Optimized T2A sequence: https://doi.org/10.6084/m9.figshare.13636142.v79.
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS) (#137864, https://www.addgene.org/137864/) was updated with (1) improved Kozak consensus sequence, (2) a slightly different codon usage in the 5' linker of the T2A, (3) a silently mutated XhoI site in iCre was reverted, and (4) four nucleotides were restored to the 5' of the WPRE.
Proper citation: RRID:Addgene_177308 Copy
Vector Backbone Description: Backbone Size:4320; Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Comments: Please note: Plasmid contains a C/T mixed base causing possible A50V mutation in URA3. This discrepancy is not known to affect plasmid function.
Proper citation: RRID:Addgene_177269 Copy
Species: Bacillus circulans
Genetic Insert: Chitinase A1 Chitin Binding Domain
Vector Backbone Description: Backbone Size:5365; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437438v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_169211 Copy
Species: Homo sapiens
Genetic Insert: ARID3A-3'UTR-Mut
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169296 Copy
Species: SARS-Cov2
Genetic Insert: C-term frag. S protein after the furin cleavag
Vector Backbone Description: Vector Backbone:pmCellFree_KA1; Vector Types:Cell-free expression vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169404 Copy
Species: Culex quinquefasciatus
Genetic Insert: pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:3931; Vector Backbone:pCR2.1; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34017003
Proper citation: RRID:Addgene_169369 Copy
Species: Arabidopsis thaliana
Genetic Insert: auxin-inducible degron (aid)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169360 Copy
Species: Arabidopsis thaliana
Genetic Insert: auxin-inducible degron (aid)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169361 Copy
Genetic Insert: pLeft
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169409 Copy
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