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Species: E. coli
Genetic Insert: Mechanosensitive Channel of Small Conductance
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5364; Vector Backbone:pET28b; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12446901
Proper citation: RRID:Addgene_78555 Copy
Genetic Insert: BFP
Vector Backbone Description: Backbone Marker:Addgene 52962; Backbone Size:12859; Vector Backbone:Lenti-Cas9-blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28253259
Proper citation: RRID:Addgene_78545 Copy
Species: Homo sapiens
Genetic Insert: gRNA1+scaffold+H1promoter+gRNA2
Vector Backbone Description: Vector Backbone:pDECKO_mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28253259
Comments: Experimental plasmid.
Proper citation: RRID:Addgene_78540 Copy
Species: Homo sapiens
Genetic Insert: gRNA1+scaffold+H1promoter+gRNA2
Vector Backbone Description: Vector Backbone:pDECKO_mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28253259
Comments: Experimental plasmid.
Proper citation: RRID:Addgene_78543 Copy
Genetic Insert: T2A-GFP
Vector Backbone Description: Backbone Marker:Addgene 52962; Backbone Size:12859; Vector Backbone:Lenti-Cas9-blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28253259
Comments: Note: Compared to the provided full plasmid sequence, Addgene's quality control sequencing finds a G202S amino acid residue substitution in the EGFP fluorophore. The depositing laboratory is aware of this residue change, and it does not affect fluorophore function.
Note that, according to the depositing laboratory, this plasmid results in a weak GFP signal that may be difficult to see by microscope, but it is useful for flow cytometry.
Proper citation: RRID:Addgene_78548 Copy
Species: Danio rerio
Genetic Insert: kdrl promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2600; Vector Backbone:pDONRp4p1r; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17306248
Proper citation: RRID:Addgene_78687 Copy
Species: Homo sapiens
Genetic Insert: MKRN1
Vector Backbone Description: Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19536131
Proper citation: RRID:Addgene_78757 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26183061
Proper citation: RRID:Addgene_78776 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Vector Backbone:pcDNA3-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26183061
Proper citation: RRID:Addgene_78777 Copy
Species: Mus musculus
Genetic Insert: PPAR_2
Vector Backbone Description: Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336050
Proper citation: RRID:Addgene_78773 Copy
Species: Homo sapiens
Genetic Insert: RIPK3
Vector Backbone Description: Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26900751
Proper citation: RRID:Addgene_78804 Copy
Vector Backbone Description: Backbone Size:14849; Vector Backbone:pLentiCRISPRV2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33272936
Proper citation: RRID:Addgene_78852 Copy
Species: Homo sapiens
Genetic Insert: RIPK1
Vector Backbone Description: Vector Backbone:pcDNA3-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26900751
Proper citation: RRID:Addgene_78842 Copy
Species: Synthetic
Genetic Insert: VC3AI
Vector Backbone Description: Backbone Size:7378; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23857461
Comments: Usage information based on sensor VC3AI: VC3AI has no or little background fluorescence, and its activation depends on the cleavage of sensor sequence of DEVDG. In fact, the sensors containing DXXDG other than DEVDG do not work. However, cells expressing VC3AI may have “background” fluorescence in some conditions, and we can conform the so-called basal fluorescence still results from the cleaved VC3AI, because cells could have a low level of activated caspase3/7 in some conditions depending on cell line and culture condition.
To avoid the possible detectable basal fluorescence, we infect cells with different dilutions of lentivirus and use the stable cell line expressing sensor protein as high as possible but without basal fluorescence for experiments. Notably, the low level of sensor protein expression work poorly. During our investigation, the basal fluorescence often happens on GC3AI, if the sensor protein expression level is high. This is because GC3AI protein is more stable than other sensors.
Proper citation: RRID:Addgene_78907 Copy
Genetic Insert: dCas9-VP64
Vector Backbone Description: Vector Backbone:pAWG; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27214048
Proper citation: RRID:Addgene_78901 Copy
Species: Homo sapiens
Genetic Insert: dCas9-VPR
Vector Backbone Description: Backbone Marker:Perrimon Lab; Backbone Size:9690; Vector Backbone:pWalium20; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26245833
Proper citation: RRID:Addgene_78897 Copy
Genetic Insert: dCas9-10XGCN4
Vector Backbone Description: Vector Backbone:pAWG; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27214048
Proper citation: RRID:Addgene_78899 Copy
Vector Backbone Description: Backbone Marker:Zhao Lab; Backbone Size:8000; Vector Backbone:pHDE; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27208253
Proper citation: RRID:Addgene_78932 Copy
Vector Backbone Description: Backbone Marker:Broad Insitute; Backbone Size:8402; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; P2A Self Cleaving Peptide; Turbo RFP reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30795624
Proper citation: RRID:Addgene_78933 Copy
Species: Homo sapiens
Genetic Insert: RIPK3
Vector Backbone Description: Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26900751
Proper citation: RRID:Addgene_78827 Copy
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