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Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Proper citation: RRID:Addgene_230743 Copy
Species: Prevotella sp. P5-125
Genetic Insert: dPspCas13b-4EHP-NES-mCherry
Vector Backbone Description: Vector Backbone:pCAGEN; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38467613
Proper citation: RRID:Addgene_233610 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Proper citation: RRID:Addgene_230694 Copy
Species: Synthetic
Genetic Insert: FlpO
Vector Backbone Description: Backbone Size:1725; Vector Backbone:pUC19; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42062565
Comments: PCR template for generating linear template for in vitro transcription. 5' and 3' beta globin UTR sequences were based on previous literature for effective modRNA expression in hiPSCs: 10.1016/j.crmeth.2022.100290
The primers used for generating PCR template also came from this work, and are as follows:
FWD: 5’- AGCTATAATACGACTCACTATAAGctcctgggcaacgtgctg-3’, which encodes the T7 promoter compatible with AG CleanCap reagent (upper-case bases) and binds to the 5’ UTR (lower-case bases)
REV: 5’-poly(T)116-GCAATGAAAATAAATGTTTTTTATTAGGCAGAAT-3’}, which encodes the poly(A) tail and binds to the 3’-UTR.
Proper citation: RRID:Addgene_232752 Copy
Species: Homo sapiens
Genetic Insert: PELO
Vector Backbone Description: Vector Backbone:pXPR_BRD051_dCas9-KRAB-MeCP2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39910293
Proper citation: RRID:Addgene_229018 Copy
Species: Synthetic
Genetic Insert: FlpO
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38915722
Proper citation: RRID:Addgene_230597 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 solvvol W
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231878 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 P+
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231872 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 R3
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231874 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 L113A+
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231873 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 E2K2
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231868 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 K5
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231866 Copy
Species: Other
Genetic Insert: T7-SP6-BC(p11-14)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: Also contains a CAG-driven EGFP
Proper citation: RRID:Addgene_231348 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Proper citation: RRID:Addgene_230775 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 E3
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231863 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231862 Copy
Species: Other
Genetic Insert: T7/T3-BC(p1-14)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40113953
Comments: The left ITR in this construct contains a 22bp deletion. We have not observed this change to impact vector production or function.
Also contains a CAG-driven EGFP with nuclear localization signals
Proper citation: RRID:Addgene_231345 Copy
Genetic Insert: YjdC
Vector Backbone Description: Backbone Size:4671; Vector Backbone:pET-14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39862855
Proper citation: RRID:Addgene_233276 Copy
Species: Chimera
Genetic Insert: mSox2 HMG flanked with N- and C-termini of Salhel-I
Vector Backbone Description: Vector Backbone:pHAGE2-TetO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39543096
Proper citation: RRID:Addgene_231533 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403729
Proper citation: RRID:Addgene_230712 Copy
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