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Species: Homo sapiens
Genetic Insert: UBB
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29463763
Proper citation: RRID:Addgene_110766 Copy
Species: Homo sapiens
Genetic Insert: ISG15
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29463763
Proper citation: RRID:Addgene_110764 Copy
Species: Homo sapiens
Genetic Insert: UBB
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29463763
Proper citation: RRID:Addgene_110767 Copy
Species: Homo sapiens
Genetic Insert: NOS2
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41395); Backbone Size:9396; Vector Backbone:pLIX_403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28427998
Proper citation: RRID:Addgene_110800 Copy
Species: Homo sapiens
Genetic Insert: UBB
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29133469
Proper citation: RRID:Addgene_110756 Copy
Species: Homo sapiens
Genetic Insert: USP30
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28945249
Proper citation: RRID:Addgene_110744 Copy
Species: Homo sapiens
Genetic Insert: USP30
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28945249
Proper citation: RRID:Addgene_110747 Copy
Species: Homo sapiens
Genetic Insert: USP30
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28945249
Proper citation: RRID:Addgene_110745 Copy
Species: Homo sapiens
Genetic Insert: USP30
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28945249
Proper citation: RRID:Addgene_110746 Copy
Species: Homo sapiens
Genetic Insert: NUFIP1
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700228
Proper citation: RRID:Addgene_110950 Copy
Species: Homo sapiens
Genetic Insert: NUFIP1 W40A
Vector Backbone Description: Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29700228
Proper citation: RRID:Addgene_110951 Copy
Species: Homo sapiens
Genetic Insert: shOGDH
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28396387
Comments: Recombination can be minimized by using NEB Stable E. coli strain grown at 30C to amplify the plasmid DNA. Even so, low recombination rate may still happen and when you eventually amplify the DNA you may need to screen through several colonies and perform diagnostic digests to identify the correct ones.
Proper citation: RRID:Addgene_110941 Copy
Species: Homo sapiens
Genetic Insert: OGDH wobble cDNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9387; Vector Backbone:pLenti6.3/V5-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28396387
Proper citation: RRID:Addgene_110938 Copy
Species: Homo sapiens
Genetic Insert: TRX1
Vector Backbone Description: Vector Backbone:pLKO (puromycin); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20074557
Comments: The Rai lab uses XL-10 gold ultracompetent cells as the growth strain, but DH5alpha should work fine too.
Proper citation: RRID:Addgene_110921 Copy
Species: Homo sapiens
Genetic Insert: MTH1
Vector Backbone Description: Backbone Marker:PUBMED ID: 19177017; Vector Backbone:pLKO-Tet-on (puromycin); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25023700
Comments: The Rai lab uses XL-10 gold ultracompetent cells as the growth strain, but DH5alpha should work fine too
Proper citation: RRID:Addgene_110918 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Vector Backbone:pET-30a based pET-pro-siRNA-V2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29873060
Proper citation: RRID:Addgene_111089 Copy
Species: Homo sapiens
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Vector Backbone:pLentiXI; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30054595
Comments: Consensus CDS were PCR amplified from gBlocks or from cDNA clones and Gibson cloned into pLentiX1
Proper citation: RRID:Addgene_111091 Copy
Species: Homo sapiens
Genetic Insert: IKK-2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9346484
Proper citation: RRID:Addgene_11105 Copy
Species: Homo sapiens
Genetic Insert: HELQ gRNA
Vector Backbone Description: Vector Backbone:pCR1068; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30054595
Proper citation: RRID:Addgene_111111 Copy
Species: Homo sapiens
Genetic Insert: RAD51B gRNA
Vector Backbone Description: Vector Backbone:pCR1068; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30054595
Proper citation: RRID:Addgene_111112 Copy
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