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Species: Drosophila melanogaster
Genetic Insert: phiC31 attB, hsp70::w
Vector Backbone Description: Backbone Size:2881; Vector Backbone:pUC; Vector Types:Drosophila transgenesis; Bacterial Resistance:Ampicillin
Comments: w[+] is hsp70 basal promoter; gives full w+ (red eyes), without heat shock.
Proper citation: RRID:Addgene_30326 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaspeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Proper citation: RRID:Addgene_26859 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26856 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26855 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. Myc-tagged STOPS lacking the SOCS box was
subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26857 Copy
Species: Drosophila melanogaster
Genetic Insert: no receptor potential A
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express norpA under control of the ninaE promoter, full length norpA cDNA was excised from pHKX-norpA and subcloned into pCNX
Proper citation: RRID:Addgene_26854 Copy
Species: Drosophila melanogaster
Genetic Insert: Gr66 gustatory receptor
Vector Backbone Description: Backbone Size:0; Vector Backbone:pP[UAS-myc]; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16979558
Proper citation: RRID:Addgene_26861 Copy
Species: Drosophila melanogaster
Genetic Insert: Gr66 gustatory receptor
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSper4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16979558
Proper citation: RRID:Addgene_26860 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4A
Vector Backbone Description: Backbone Size:3990; Vector Backbone:pRmHa3-CHA2FL2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Proper citation: RRID:Addgene_20232 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4A
Vector Backbone Description: Backbone Size:3990; Vector Backbone:pRmHa3-CHA2FL2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Comments: D153E mutation is also present, but it does affect the plasmid.
Proper citation: RRID:Addgene_20235 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4B
Vector Backbone Description: Backbone Size:5608; Vector Backbone:pBacPAK-HFT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Comments: TEV tag ENPYFQG instead of ENLYFQG.
The cDNA used in making dKDM4B(CG33182) constructs has incomplete 3' end. It is because NCBI data has updated the sequence after I made the construct. There is a comment on NCBI page https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=161077032&from=148&to=1920&view=gbwithparts indicates that they replaced a former sequence of CG33182 https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=28573833 with the current 1773bp one.
Proper citation: RRID:Addgene_20234 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4A
Vector Backbone Description: Backbone Size:5608; Vector Backbone:pBacPAK-HFT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Comments: TEV tag ENPYFQG instead of ENLYFQG.
Proper citation: RRID:Addgene_20233 Copy
Species: Drosophila melanogaster
Genetic Insert: spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19029882
Comments: There are several polymorphisms in the 3’UTR of the expression construct and should not affect the function.
Proper citation: RRID:Addgene_20163 Copy
Species: Drosophila melanogaster
Genetic Insert: Gr64d
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19026541
Proper citation: RRID:Addgene_21079 Copy
Species: Drosophila melanogaster
Genetic Insert: Gr64a
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17715294
Proper citation: RRID:Addgene_21081 Copy
Species: Drosophila melanogaster
Genetic Insert: Pavarotti
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4200; Vector Backbone:pMT-GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19720876
Comments: This plasmid was originally generated by Dr. Gohta Goshima and published in MBOC. Vol. 16, 3896–3907.
Proper citation: RRID:Addgene_24286 Copy
Species: Drosophila melanogaster
Genetic Insert: p150 Dynactin
Vector Backbone Description: Backbone Marker:Drosophila Gateway Vector Collection; Backbone Size:5943; Vector Backbone:pMT-GW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17576797
Comments: Dr. Goshima made a C-terminal GFP p150 plasmid and Vale lab used his pENTR vector to make an N-terminal fusion using Gateway cloning.
Y1238H mutation detected in Addgene's BGH-rev sequence is not likely to affect function.
Proper citation: RRID:Addgene_24283 Copy
Species: Drosophila melanogaster
Genetic Insert: Shibire (ts1)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: Shibire was PCR amplified from genomic DNA of UAS-shibire transgenic flies (Kitamoto, 2001) using genUASFOR and genUASREVsv40 oligos, and ligated into the NotI/KpnI sites of pQUAST.
Proper citation: RRID:Addgene_24362 Copy
Species: Drosophila melanogaster
Genetic Insert: Shibire (ts1)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pQUAS>stop>mCD8-GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The NotI fragment containing the mCD8-GFP cassette was excised from pQUAS>stop>mCD8-
GFP, the vector was blunted, and ligated to a blunted NotI/KpnI shibirets1 isolated from pQUAST-shibirets1. ts1
Proper citation: RRID:Addgene_24363 Copy
Species: Drosophila melanogaster
Genetic Insert: HPat
Vector Backbone Description: Backbone Marker:Elisa Izaurralde; Backbone Size:6080; Vector Backbone:pAc5.1B-EGFP; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20404111
Proper citation: RRID:Addgene_25029 Copy
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