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Species: Homo sapiens
Genetic Insert: protein kinase R
Vector Backbone Description: Vector Backbone:pet11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15567412
Proper citation: RRID:Addgene_42934 Copy
Species: Perch (Morone americana)
Genetic Insert: Connexin 35
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4990; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15201336
Comments: Subcloned from cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637.
Proper citation: RRID:Addgene_42920 Copy
Species: A. victoria
Genetic Insert: Aquamarine
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23192565
Proper citation: RRID:Addgene_42888 Copy
Species: A. victoria
Genetic Insert: Aquamarine
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4751; Vector Backbone:pProEX HTa; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23192565
Proper citation: RRID:Addgene_42889 Copy
Genetic Insert: CRISPR-BsaI
Vector Backbone Description: Backbone Size:2254; Vector Backbone:pZE21-MCS1; Vector Types:Bacterial Expression, CRISPR, E.coli; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23360965
Comments: This plasmid is also known as pDB129.
This plasmid contains a duplicated spacer insertion site (4 BsaI sites instead of 2). Please note that these extra sites are eliminated upon treatment with BsaI, and the depositing lab has used this version extensively without issue. Addgene has found that versions of the plasmid with and without the duplicated spacer are present, both are predicted to perform similarly.
The HME63 strain is available from the from the Court Lab:
http://redrecombineering.ncifcrf.gov/strains.html
For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/
Proper citation: RRID:Addgene_42875 Copy
Species: Rattus norvegicus
Genetic Insert: Red Fluorescent Calcium binding Protein
Vector Backbone Description: Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2753; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23459413
Comments: Gene was cloned using BaMHI and HindIII - But part of the vector backbone then becomes part of the protein (leader sequence containing histag) - using NdeI and HindIII will completely remove the open reading frame/gene.
Proper citation: RRID:Addgene_42874 Copy
Genetic Insert: L7.5.1
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pCT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602401
Proper citation: RRID:Addgene_42900 Copy
Species: Mus musculus
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Compared to GenBank accession number L06039.1, the PECAM-1 sequence in this plasmid contains 2 nucleotide mismatches in the 5' untranslated region (UTR).
Proper citation: RRID:Addgene_42901 Copy
Species: Homo sapiens
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42902 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-RhoA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3991; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23377377
Proper citation: RRID:Addgene_42958 Copy
Species: Homo sapiens
Genetic Insert: Negative control Functionally Dead Human L1 element
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16507671
Comments: These plasmids consist of the L1RP element tagged with an enhanced green fluorescent protein (EGFP) cassette (pL1RP-EGFP) in a pCEP4 backbone (Invitrogen). The L1 element is driven by its 5′-UTR and an upstream CMV MIE promoter. L1-EGFP (EF06R, Addgene plasmid #42940) was derived from L1RP-EGFP by blunt ligation of 1–1392 nt of pPur (BD/Clontech) into the NruI site of pCEP4.
A negative control ‘dead’ L1-EGFP (EF05J, Addgene plasmid #42941) was similarly derived from pL1RP(JM111)-EGFP, which contains disabling mutations in ORF1.
An EN− plasmid (EF13E, Addgene plasmid #42942) was created by swapping 1927–3708 nt (Age I–Bcl I) from JM102D205A, which contains an endonuclease function-abrogating D205A point mutation in the EN domain, into EF06R.
A control plasmid (EF12J, Addgene plasmid #42943) was similarly derived from JM102. JM102 and JM102D205A are derived from L1.3, which differs from L1RP by 10 base changes in the region swapped.
The A250V mutation found during Addgene QC is conservative and should not affect function.
Proper citation: RRID:Addgene_42941 Copy
Species: Homo sapiens
Genetic Insert: Human L1 element
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16507671
Comments: These plasmids consist of the L1RP element tagged with an enhanced green fluorescent protein (EGFP) cassette (pL1RP-EGFP) in a pCEP4 backbone (Invitrogen). The L1 element is driven by its 5′-UTR and an upstream CMV MIE promoter. L1-EGFP (EF06R, Addgene plasmid #42940) was derived from L1RP-EGFP by blunt ligation of 1–1392 nt of pPur (BD/Clontech) into the NruI site of pCEP4.
A negative control ‘dead’ L1-EGFP (EF05J, Addgene plasmid #42941) was similarly derived from pL1RP(JM111)-EGFP, which contains disabling mutations in ORF1.
An EN− plasmid (EF13E, Addgene plasmid #42942) was created by swapping 1927–3708 nt (Age I–Bcl I) from JM102D205A, which contains an endonuclease function-abrogating D205A point mutation in the EN domain, into EF06R.
A control plasmid (EF12J, Addgene plasmid #42943) was similarly derived from JM102. JM102 and JM102D205A are derived from L1.3, which differs from L1RP by 10 base changes in the region swapped.
I->T substitution at the end of ORF 2 should not affect plasmid function.
Proper citation: RRID:Addgene_42940 Copy
Species: Mus musculus
Genetic Insert: p160 myb binding protein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14744933
Proper citation: RRID:Addgene_43 Copy
Species: Synthetic
Genetic Insert: I-AACT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42970 Copy
Species: Synthetic
Genetic Insert: II-AAAC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43220 Copy
Species: Synthetic
Genetic Insert: II-CGTT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43330 Copy
Species: Synthetic
Genetic Insert: II-CTCA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43335 Copy
Species: Synthetic
Genetic Insert: III-CACC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43544 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Joung lab MLM3636; Vector Backbone:Human-gRNA-ExpressionVector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Human cell gRNA Expression Vector for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9). See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
Proper citation: RRID:Addgene_43860 Copy
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43859 Copy
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