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Species: Caenorhabditis elegans
Genetic Insert: sqt-1(e1350) gRNA2
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_59935 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59788 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59787 Copy
Species: Caenorhabditis elegans
Genetic Insert: ZF1 domain from pie-1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pBR322; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59790 Copy
Species: Caenorhabditis elegans
Genetic Insert: rde-1(D718) gRNA
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_59927 Copy
Species: Caenorhabditis elegans
Genetic Insert: dhc-3
Vector Backbone Description: Backbone Marker:Andrew Fire; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25066299
Comments: Source BioScience LifeSciences C. elegans RNAi library clones utilized in plasmid construction (Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M. and Ahringer, J. (2000) Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330.)
Proper citation: RRID:Addgene_59997 Copy
Species: Caenorhabditis elegans
Genetic Insert: dhc-3
Vector Backbone Description: Backbone Marker:Andrew Fire; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25066299
Comments: Source BioScience LifeSciences C. elegans RNAi library clones utilized in plasmid construction (Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M. and Ahringer, J. (2000) Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330.)
Proper citation: RRID:Addgene_59996 Copy
Species: Caenorhabditis elegans
Genetic Insert: sqt-1(e1350) gRNA1
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_60212 Copy
Species: Caenorhabditis elegans
Genetic Insert: cha-1
Vector Backbone Description: Vector Backbone:pPD114.108; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081161
Proper citation: RRID:Addgene_78191 Copy
Species: Caenorhabditis elegans
Genetic Insert: cha-1
Vector Backbone Description: Vector Backbone:pPD114.108; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081161
Proper citation: RRID:Addgene_78193 Copy
Species: Caenorhabditis elegans
Genetic Insert: fil-2
Vector Backbone Description: Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19416714
Proper citation: RRID:Addgene_78188 Copy
Species: Caenorhabditis elegans
Genetic Insert: sls-1.2
Vector Backbone Description: Backbone Marker:Transgen; Backbone Size:3830; Vector Backbone:pEASY Blunt Simple Cloning Kit; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27557708
Proper citation: RRID:Addgene_79003 Copy
Species: Caenorhabditis elegans
Genetic Insert: sls-1.2
Vector Backbone Description: Backbone Marker:Transgen; Backbone Size:3830; Vector Backbone:pEASY Blunt Simple Cloning Kit; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27557708
Proper citation: RRID:Addgene_79002 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::lin-26 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80357 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::ets-4 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80355 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::cul-4 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80353 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80352 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3::gfp::h2b::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044730
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_65631 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APs12
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65597 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044730
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_65632 Copy
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