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Species: Rattus norvegicus
Genetic Insert: Nrxn1beta AS4(-)
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI).
Insert can also be released by single digest using XhoI.
The rat neurexin 1 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the region homologous to alternatively spliced segment 5 (AS5) of mouse neurexin 1 beta (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58267 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1alpha
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest).
The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58266 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: Please see Addgene plasmid 40755 for more information on GCaMP6f; also note that GCaMP6f was published in: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.)
Proper citation: RRID:Addgene_58514 Copy
Species: Rattus norvegicus
Genetic Insert: alpha2 delta-1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22678293
Proper citation: RRID:Addgene_58730 Copy
Species: Rattus norvegicus
Genetic Insert: C2AB-linker-SNAP-25_C
Vector Backbone Description: Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26280336
Proper citation: RRID:Addgene_70057 Copy
Species: Rattus norvegicus
Genetic Insert: pHoenix
Vector Backbone Description: Backbone Size:8400; Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26551543
Proper citation: RRID:Addgene_70111 Copy
Species: Rattus norvegicus
Genetic Insert: GAT
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12223478
Proper citation: RRID:Addgene_70107 Copy
Species: Rattus norvegicus
Genetic Insert: snca
Vector Backbone Description: Vector Backbone:pAAV-D(+)-U6-siRNA-CMV-GFP; Vector Types:Mammalian Expression, AAV, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26075822
Proper citation: RRID:Addgene_75437 Copy
Species: Rattus norvegicus
Genetic Insert: Homer1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Membrane Adaptor
Proper citation: RRID:Addgene_54120 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Rat Beta-2 Connexin 26. Excitation = 487; Emission = 509. Localization data: Gap Junctions
Proper citation: RRID:Addgene_54053 Copy
Species: Rattus norvegicus
Genetic Insert: Lysosomes
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: LAMP1 - Lysosome Membrane
Proper citation: RRID:Addgene_54149 Copy
Species: Rattus norvegicus
Genetic Insert: RatLC3B
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22635056
Comments: Microtubule-associated proteins 1A and 1B light chain 3. Excitation = 487; Emission = 509. Localization data: Microtubule-Binding Protein
Proper citation: RRID:Addgene_54147 Copy
Species: Rattus norvegicus
Genetic Insert: PSD95
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Membrane Scaffold
Proper citation: RRID:Addgene_54238 Copy
Species: Rattus norvegicus
Genetic Insert: sEspin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Actin Bundling
Proper citation: RRID:Addgene_54254 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22635056
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54881 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54880 Copy
Species: Rattus norvegicus
Genetic Insert: PSD95
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54941 Copy
Species: Rattus norvegicus
Genetic Insert: CathespinB
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54871 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1 phLuorin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Lysosomes-20 double labeled with phLuorin. Excitation = 568; Emission = 592
LAMP1 TM domain corresponds to aa 370-407 of LAMP1 (NM_012857.2).
Proper citation: RRID:Addgene_54918 Copy
Species: Rattus norvegicus
Genetic Insert: CathespinB
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55007 Copy
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