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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pPET-PKR/PPase
 
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1+ mentions
RRID:Addgene_42934 protein kinase R Homo sapiens Ampicillin PMID:15567412 Vector Backbone:pet11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:58 5
PCx35-pcDNA
 
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RRID:Addgene_42920 Connexin 35 Perch (Morone americana) Ampicillin PMID:15201336 Subcloned from cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637. Backbone Marker:Invitrogen; Backbone Size:4990; Vector Backbone:pcDNA3.1 Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:58 1
pAquaN1
 
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RRID:Addgene_42888 Aquamarine A. victoria Kanamycin PMID:23192565 Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T66S and H149G 2026-08-15 01:14:58 3
pPROEX Aqua
 
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RRID:Addgene_42889 Aquamarine A. victoria Ampicillin PMID:23192565 Backbone Marker:Life Technologies; Backbone Size:4751; Vector Backbone:pProEX HTa; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T65S and H148G 2026-08-15 01:14:58 3
pCRISPR
 
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10+ mentions
RRID:Addgene_42875 CRISPR-BsaI Kanamycin PMID:23360965 This plasmid is also known as pDB129. This plasmid contains a duplicated spacer insertion site (4 BsaI sites instead of 2). Please note that these extra sites are eliminated upon treatment with BsaI, and the depositing lab has used this version extensively without issue. Addgene has found that versions of the plasmid with and without the duplicated spacer are present, both are predicted to perform similarly. The HME63 strain is available from the from the Court Lab: http://redrecombineering.ncifcrf.gov/strains.html For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/ Backbone Size:2254; Vector Backbone:pZE21-MCS1; Vector Types:Bacterial Expression, CRISPR, E.coli; Bacterial Resistance:Kanamycin 2026-08-15 01:14:58 31
pRSET-RCaMP1h
 
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RRID:Addgene_42874 Red Fluorescent Calcium binding Protein Rattus norvegicus Ampicillin PMID:23459413 Gene was cloned using BaMHI and HindIII - But part of the vector backbone then becomes part of the protein (leader sequence containing histag) - using NdeI and HindIII will completely remove the open reading frame/gene. Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2753; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:58 7
pCT-L7.5.1
 
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RRID:Addgene_42900 L7.5.1 Ampicillin PMID:18602401 Backbone Size:6200; Vector Backbone:pCT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:58 3
pcDNAI Neo mouse PECAM FL
 
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RRID:Addgene_42901 PECAM Mus musculus Kanamycin Compared to GenBank accession number L06039.1, the PECAM-1 sequence in this plasmid contains 2 nucleotide mismatches in the 5' untranslated region (UTR). Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:58 1
pcDNAI Neo human PECAM FL
 
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RRID:Addgene_42902 PECAM Homo sapiens Kanamycin Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:58 1
pCry2PHR-mCh-RhoA
 
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RRID:Addgene_42958 Cry2PHR-mCherry-RhoA Homo sapiens Kanamycin PMID:23377377 Backbone Marker:Clontech; Backbone Size:3991; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:58 1
EF05J
 
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1+ mentions
RRID:Addgene_42941 Negative control Functionally Dead Human L1 element Homo sapiens Ampicillin PMID:16507671 These plasmids consist of the L1RP element tagged with an enhanced green fluorescent protein (EGFP) cassette (pL1RP-EGFP) in a pCEP4 backbone (Invitrogen). The L1 element is driven by its 5′-UTR and an upstream CMV MIE promoter. L1-EGFP (EF06R, Addgene plasmid #42940) was derived from L1RP-EGFP by blunt ligation of 1–1392 nt of pPur (BD/Clontech) into the NruI site of pCEP4. A negative control ‘dead’ L1-EGFP (EF05J, Addgene plasmid #42941) was similarly derived from pL1RP(JM111)-EGFP, which contains disabling mutations in ORF1. An EN− plasmid (EF13E, Addgene plasmid #42942) was created by swapping 1927–3708 nt (Age I–Bcl I) from JM102D205A, which contains an endonuclease function-abrogating D205A point mutation in the EN domain, into EF06R. A control plasmid (EF12J, Addgene plasmid #42943) was similarly derived from JM102. JM102 and JM102D205A are derived from L1.3, which differs from L1RP by 10 base changes in the region swapped. The A250V mutation found during Addgene QC is conservative and should not affect function. Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin ORF1 R261A, R262A 2026-08-15 01:14:58 1
EF06R
 
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1+ mentions
RRID:Addgene_42940 Human L1 element Homo sapiens Ampicillin PMID:16507671 These plasmids consist of the L1RP element tagged with an enhanced green fluorescent protein (EGFP) cassette (pL1RP-EGFP) in a pCEP4 backbone (Invitrogen). The L1 element is driven by its 5′-UTR and an upstream CMV MIE promoter. L1-EGFP (EF06R, Addgene plasmid #42940) was derived from L1RP-EGFP by blunt ligation of 1–1392 nt of pPur (BD/Clontech) into the NruI site of pCEP4. A negative control ‘dead’ L1-EGFP (EF05J, Addgene plasmid #42941) was similarly derived from pL1RP(JM111)-EGFP, which contains disabling mutations in ORF1. An EN− plasmid (EF13E, Addgene plasmid #42942) was created by swapping 1927–3708 nt (Age I–Bcl I) from JM102D205A, which contains an endonuclease function-abrogating D205A point mutation in the EN domain, into EF06R. A control plasmid (EF12J, Addgene plasmid #42943) was similarly derived from JM102. JM102 and JM102D205A are derived from L1.3, which differs from L1RP by 10 base changes in the region swapped. I->T substitution at the end of ORF 2 should not affect plasmid function. Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:58 7
pcDNA-p160(1-200)
 
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RRID:Addgene_43 p160 myb binding protein Mus musculus Ampicillin PMID:14744933 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:59 4
I-AACT
 
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RRID:Addgene_42970 I-AACT Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:14:58 1
II-AAAC
 
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RRID:Addgene_43220 II-AAAC Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:15:02 1
II-CGTT
 
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1+ mentions
RRID:Addgene_43330 II-CGTT Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:15:03 6
II-CTCA
 
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1+ mentions
RRID:Addgene_43335 II-CTCA Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:15:03 2
III-CACC
 
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RRID:Addgene_43544 III-CACC Synthetic Ampicillin and Kanamycin PMID:23246482 Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:15:06 1
MLM3636
 
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100+ mentions
RRID:Addgene_43860 None Ampicillin Human cell gRNA Expression Vector for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9). See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details. Backbone Marker:Joung lab MLM3636; Vector Backbone:Human-gRNA-ExpressionVector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:10 142
pTAL_RFP
 
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RRID:Addgene_43859 Ampicillin PMID:23246482 Backbone Size:4400; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:10 2

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