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Species: Danio rerio
Genetic Insert: Flrt3 mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20525357
Comments: See map and Genbank file for plasmid details.
Proper citation: RRID:Addgene_28217 Copy
Species: Danio rerio
Genetic Insert: Unc5b mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20525357
Comments: See map and Genbank file for plasmid details.
Please note that Addgene's sequencing results identified 4 single nucleotide mismatches (at bp# 3373, 3376, 3380 and 3386) when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.
Proper citation: RRID:Addgene_28214 Copy
Species: Danio rerio
Genetic Insert: pBS119d10L
Vector Backbone Description: Backbone Marker:Strategene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12167406
Comments: To view partial vector sequence from the depositing lab, please click on the Reviews link.
Proper citation: RRID:Addgene_22081 Copy
Species: Danio rerio
Genetic Insert: vegf
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110173
Proper citation: RRID:Addgene_22417 Copy
Species: Danio rerio
Genetic Insert: VSP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_23959 Copy
Species: Danio rerio
Genetic Insert: VSP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_23960 Copy
Species: Danio rerio
Genetic Insert: H2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.
*H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.
Proper citation: RRID:Addgene_24334 Copy
Species: Danio rerio
Genetic Insert: Rab8B
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences.
Proper citation: RRID:Addgene_24908 Copy
Species: Danio rerio
Genetic Insert: fishRab8B
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences.
Proper citation: RRID:Addgene_24907 Copy
Species: Danio rerio
Genetic Insert: pCS4+-P2A
Vector Backbone Description: Backbone Size:4146; Vector Backbone:pCS4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21602908
Proper citation: RRID:Addgene_31566 Copy
Species: Danio rerio
Genetic Insert: TAL array targeting gria3a
Vector Backbone Description: Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT
Proper citation: RRID:Addgene_32283 Copy
Species: Danio rerio
Genetic Insert: TAL array targeting gria3a
Vector Backbone Description: Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT
Proper citation: RRID:Addgene_32281 Copy
Species: Danio rerio
Genetic Insert: TAL array targeting gria3a
Vector Backbone Description: Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT
Proper citation: RRID:Addgene_32282 Copy
Species: Danio rerio
Genetic Insert: TAL array targeting hey2
Vector Backbone Description: Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822241
Comments: TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT
Proper citation: RRID:Addgene_32279 Copy
Species: Danio rerio
Genetic Insert: F1 GAC
Vector Backbone Description: Backbone Size:6141; Vector Backbone:pCS2 (modified); Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30694 Copy
Species: Danio rerio
Genetic Insert: F1 GCG
Vector Backbone Description: Backbone Size:4789; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30699 Copy
Species: Danio rerio
Genetic Insert: F1 GCC
Vector Backbone Description: Backbone Size:6141; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30698 Copy
Species: Danio rerio
Genetic Insert: F2 CAG
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30717 Copy
Species: Danio rerio
Genetic Insert: F2 AGA
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30756 Copy
Species: Danio rerio
Genetic Insert: F3 AGA
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_30757 Copy
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