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Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79021 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79020 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78995 Copy
Species: Chlorocebus pygerythrus pygerythrus
Genetic Insert: OSF-TRIM5alpha(African green monkey pygerythrus)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Comments: Note that the TRIM5 insert in this plasmid contains a V434A polymorphism compared to GenBank ID AAW72440.1. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_79031 Copy
Species: Homo sapiens
Genetic Insert: OneSTrEP-FLAG- Cyclophilin A
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4640; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79039 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78988 Copy
Species: Soybean
Genetic Insert: APEX2 anchored to the cytosolic face of the ER membrane
Vector Backbone Description: Backbone Size:7740; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25419960
Comments: Additional publication for this plasmid:
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.
Hung et. al. Elife 2017 https://www.ncbi.nlm.nih.gov/pubmed/28441135
Proper citation: RRID:Addgene_79055 Copy
Species: E.coli
Genetic Insert: AlkB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5243; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26214130
Proper citation: RRID:Addgene_79050 Copy
Species: Homo sapiens
Genetic Insert: C-terminal APEX2 fused MICU1
Vector Backbone Description: Backbone Size:7740; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25419960
Proper citation: RRID:Addgene_79057 Copy
Species: Synthetic
Genetic Insert: LbCpf1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6000; Vector Backbone:pMAL-c5X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27272384
Proper citation: RRID:Addgene_79008 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27272384
Proper citation: RRID:Addgene_78956 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27272384
Proper citation: RRID:Addgene_78957 Copy
Species: Mus musculus
Genetic Insert: R7BD
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4900; Vector Backbone:pGEX-4T-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19386765
Proper citation: RRID:Addgene_79149 Copy
Genetic Insert: eSpCas9(1.1)
Vector Backbone Description: Backbone Marker:Addgene #71814; Vector Backbone:eSpCas9(1.1); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_79145 Copy
Species: Synthetic
Genetic Insert: tBFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26830878
Comments: Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim WA. Cell. 2016 Feb 11;164(4):770-9. Epub 2016 Jan 28.
Proper citation: RRID:Addgene_79130 Copy
Species: Other
Genetic Insert: Citrine
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:11282; Vector Backbone:pMpGWB103; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24259681
Proper citation: RRID:Addgene_79137 Copy
Species: P. areuginosa
Genetic Insert: WspR
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3719; Vector Backbone:pCOLADuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27382070
Proper citation: RRID:Addgene_79163 Copy
Genetic Insert: T7-tRNA-Spinach2-Ct
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET31b+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27382070
Proper citation: RRID:Addgene_79158 Copy
Vector Backbone Description: Backbone Marker:Chiu W; Backbone Size:4180; Vector Backbone:CaMV35S- sGFP(S65T)-NOS vector; Vector Types:Plant Expression, Reporter plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20974974
Proper citation: RRID:Addgene_79183 Copy
Species: Synthetic
Genetic Insert: Adhesin Involved in Diffuse Adherence
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pKM1D; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22943700
Proper citation: RRID:Addgene_79180 Copy
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