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Species: Synthetic
Genetic Insert: ChemoY-CaM
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pET-51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193814 Copy
Species: Synthetic
Genetic Insert: ChemoR-CaM
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pET-51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193815 Copy
Species: Synthetic
Genetic Insert: ChemoG-NAD
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193821 Copy
Species: Synthetic
Genetic Insert: 47xCAG RBS"m1"-sfGFP
Vector Backbone Description: Backbone Size:2578; Vector Backbone:pRBS"m1"-sfGFP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36179672
Proper citation: RRID:Addgene_191400 Copy
Species: Synthetic
Genetic Insert: [4xCox8]-ChemoG-NAD
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193823 Copy
Species: Synthetic
Genetic Insert: ChemoB-NAD
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193824 Copy
Species: Synthetic
Genetic Insert: ChemoR-ATP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193820 Copy
Species: Synthetic
Genetic Insert: LanYFP
Vector Backbone Description: Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_201856 Copy
Species: Synthetic
Genetic Insert: Protein N
Vector Backbone Description: Backbone Size:4145; Vector Backbone:pT7-LacO; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36179672
Proper citation: RRID:Addgene_191126 Copy
Species: Synthetic
Genetic Insert: ChemoD-NAD
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193826 Copy
Species: Synthetic
Genetic Insert: ChemoL-NAD
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193827 Copy
Species: Synthetic
Genetic Insert: ChemoL-NAD-NLS3x
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37291200
Proper citation: RRID:Addgene_193828 Copy
Species: Synthetic
Genetic Insert: rCAG
Vector Backbone Description: Backbone Size:3287; Vector Backbone:pHR-Tre3G-47xCAG-12xMS2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36179672
Proper citation: RRID:Addgene_191121 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37294017
Proper citation: RRID:Addgene_197674 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37294017
Proper citation: RRID:Addgene_197672 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37294017
Proper citation: RRID:Addgene_197673 Copy
Species: Synthetic
Genetic Insert: tT7
Vector Backbone Description: Backbone Marker:John Dueber Lab, https://doi.org/10.1021/sb500366v; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37009624
Proper citation: RRID:Addgene_200154 Copy
Species: Synthetic
Genetic Insert: BCD 13K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200049 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37294017
Proper citation: RRID:Addgene_197669 Copy
Species: Synthetic
Genetic Insert: BCD 25K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200045 Copy
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