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Genetic Insert: SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33743213
Proper citation: RRID:Addgene_169468 Copy
Genetic Insert: SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33743213
Proper citation: RRID:Addgene_169463 Copy
Genetic Insert: SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33743213
Proper citation: RRID:Addgene_169464 Copy
Genetic Insert: SARS-CoV Spike truncated to remove 18 amino acids
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33743213
Proper citation: RRID:Addgene_169465 Copy
Species: Other
Genetic Insert: SARS-CoV-2 B.1.1.298 Spike truncated to remove 18 amino acids
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33743213
Proper citation: RRID:Addgene_169460 Copy
Species: Synthetic
Genetic Insert: tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:8594; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169734 Copy
Species: E.coli and partially synthetic
Genetic Insert: 1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
Vector Backbone Description: Backbone Marker:Uzzau et al (PubMed 11742086). Note, pSU311 was initially derived from pGP704 (Miller & Mekalanos; PubMed 2836362); Backbone Size:1932; Vector Backbone:pSU311; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11742086
Comments: The 1xHA-FRT-aph-FRT segment is amplified by PCR with primers ending with the sequences 5'...TATCCGTATGATGTTCCTGAT-3' (Forward primer) and 5'...CATATGAATATCCTCCTTAG-3' (Reverse primer) and carrying 40 nt 5' extensions identical to the sequence immediately preceding the stop codon of the targeted gene (Fw primer) and to a region downstream from it (Rv primer). The amplified fragment is used for DNA recombineering in a strain expressing the Lambda Red operon.
Proper citation: RRID:Addgene_169697 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MatA
Vector Backbone Description: Vector Backbone:pCR™-Blunt II-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29150593
Proper citation: RRID:Addgene_169457 Copy
Species: Xenopus laevis
Genetic Insert: Engrailed-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Xenopus Probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1679005
Comments: In situ probe cut with Xba1, transcribe with T3.
Proper citation: RRID:Addgene_16950 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA for MATα
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29150593
Proper citation: RRID:Addgene_169454 Copy
Species: E.coli and partially synthetic
Genetic Insert: 1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
Vector Backbone Description: Backbone Marker:Uzzau et al (PubMed 11742086). Note, pSU311 was initially derived from pGP704 (Miller & Mekalanos; PubMed 2836362); Backbone Size:1932; Vector Backbone:pSU311; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11742086
Comments: The 1xHA-FRT-cat-FRT segment is amplified by PCR with primers ending with the sequences 5'...TATCCGTATGATGTTCCTGAT-3' (Forward primer) and 5'...CATATGAATATCCTCCTTAG-3' (Reverse primer) and carrying 40 nt 5' extensions identical to the sequence immediately preceding the stop codon of the targeted gene (Fw primer) and to a region downstream from it (Rv primer). The amplified fragment is used for DNA recombineering in a strain expressing the Lambda Red operon.
Proper citation: RRID:Addgene_169696 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30318144
Proper citation: RRID:Addgene_169450 Copy
Vector Backbone Description: Backbone Marker:Andrew Fire; Vector Backbone:pPD158.87; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34791215
Comments: Please visit https://doi.org/10.1101/2020.11.25.398784 for bioRxiv preprint.
Proper citation: RRID:Addgene_169692 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ecm2 1-143
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5000; Vector Backbone:pRS416; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Proper citation: RRID:Addgene_169923 Copy
Vector Backbone Description: Vector Backbone:pHAtC; Vector Types:Plant Expression, Plant Binary vector; Bacterial Resistance:Streptomycin
Defining Citation: PMID:32190101
Proper citation: RRID:Addgene_169765 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ecm2 1-325
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5000; Vector Backbone:pRS416; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Proper citation: RRID:Addgene_169926 Copy
Species: Synthetic
Genetic Insert: sgRNA towards glpR in Pseudomonas putida
Vector Backbone Description: Vector Backbone:pBBR1k-GFP; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Proper citation: RRID:Addgene_169873 Copy
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the Addgene verified sequence differs from the depositor's reference sequence linked under the supplemental documents section. These differences do not alter the plasmid function.
Proper citation: RRID:Addgene_169872 Copy
Species: Homo sapiens
Genetic Insert: antiCD19-Notch1-GL4VP64
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32002793
Proper citation: RRID:Addgene_169918 Copy
Vector Backbone Description: Backbone Marker:Addgene #89753; Vector Backbone:pU6-Cj-sgRNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751124
Proper citation: RRID:Addgene_169915 Copy
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