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Species: Homo sapiens
Genetic Insert: miR-E (miR-30 variant)
Vector Backbone Description: Vector Backbone:pQCXIX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24332856
Proper citation: RRID:Addgene_111166 Copy
Species: Homo sapiens
Genetic Insert: hShh
Vector Backbone Description: Vector Backbone:pOEM1; Vector Types:Mammalian Expression, Insect Expression, Baculoviral rescue shuttle vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27120163
Proper citation: RRID:Addgene_111156 Copy
Species: Homo sapiens
Genetic Insert: potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens]
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29429937
Proper citation: RRID:Addgene_111267 Copy
Species: Homo sapiens
Genetic Insert: hTERT, p53 dominant negative
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: This plasmid was generated by cloning FLAG-hTERT cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site p53DD cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11128 Copy
Species: Homo sapiens
Genetic Insert: RalA WT
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZLblast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15950903
Proper citation: RRID:Addgene_11124 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D1, Cyclin Dependent Kinase 4
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: This plasmid was generated by cloning cyclinD1 cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site CDK4R24C cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11129 Copy
Species: Homo sapiens
Genetic Insert: human CLYBL
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pLys1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056341
Proper citation: RRID:Addgene_111290 Copy
Species: Homo sapiens
Genetic Insert: RNAi against human Protection of Telomeres 1
Vector Backbone Description: Backbone Size:3200; Vector Backbone:PSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15620654
Comments: Target sequence is: TACCT CGCAC TTCAA GCAA.
Proper citation: RRID:Addgene_11133 Copy
Species: Homo sapiens
Genetic Insert: RNAi vector for human protection of telomeres 1(control for 1660)
Vector Backbone Description: Backbone Size:3200; Vector Backbone:PSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15620654
Comments: Plasmid contains the 1660 sequence (GTACT AGAAG CCTAT CTCA), but is not a hairpin.
Proper citation: RRID:Addgene_11132 Copy
Species: Homo sapiens
Genetic Insert: RNAi vector for human protection of telomeres 1(control for 344)
Vector Backbone Description: Backbone Size:3200; Vector Backbone:PSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15620654
Comments: Plasmid contains the 344 sequence (TACCT CGCAC TTCAA GCAA), but is not a hairpin.
Proper citation: RRID:Addgene_11134 Copy
Species: Homo sapiens
Genetic Insert: human CLYBL ( aa23-340) codon optimized for bacterial expression
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29056341
Proper citation: RRID:Addgene_111288 Copy
Species: Homo sapiens
Genetic Insert: c-myc, HRas
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16267004
Comments: Contains mouse c-myc and human Hras. This plasmid was generated by cloning c-MycT58A cDNA with flanking BamHI-EcoRI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site H-RasG12V cDNA in which HindIII/ClaI sites were again added by PCR.
Proper citation: RRID:Addgene_11130 Copy
Species: Homo sapiens
Genetic Insert: hH1-ARRET
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR/D-hH1; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_111382 Copy
Species: Homo sapiens
Genetic Insert: TERRA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR/D-hH1; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27537914
Comments: Depositing Scientist Sequence may not contain all correct information about the telomere sequence repeats such as canonical and variant repeat positions, and precise information about insert length. Further, the Addgene Partial NGS Result does not include the entire region of telomere repeats. Note that the BamHI site was destroyed during cloning.
Proper citation: RRID:Addgene_111383 Copy
Species: Homo sapiens
Genetic Insert: ARRET w/o polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAd/PL-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111374 Copy
Species: Homo sapiens
Genetic Insert: ARRET polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6-UbC/V5-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_111378 Copy
Species: Homo sapiens
Genetic Insert: TERRA polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti4/V5-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27537914
Comments: Please see the Addgene Partial NGS Result for the verified backbone. Please note this result contains only a part (around 670 bps) of the 800 bps telomere sequence repeats, with further variant repeats not seen by Sanger sequencing by the Depositor.
This plasmid may be prone to recombination due to the telomere hexanucleotide repeats, and therefore screening multiple colonies for the correct insert length is recommended before proceeding with downstream work
Proper citation: RRID:Addgene_111375 Copy
Species: Homo sapiens
Genetic Insert: potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens]
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29429937
Proper citation: RRID:Addgene_111451 Copy
Species: Homo sapiens
Genetic Insert: Sox9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27566146
Comments: Reference for original WT SOX9 plasmid - https://www.ncbi.nlm.nih.gov/pubmed/?term=20651055
We used these primers for SDM Sox9 F7site5mut1F: 5'-ggcccaccggccccacccAccAcccccaaaa-3' Sox9 F7site5mut1R: 5'-ttttgggggTggTgggtggggccggtgggcc-3'
Proper citation: RRID:Addgene_111455 Copy
Species: Homo sapiens
Genetic Insert: potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens]
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29429937
Proper citation: RRID:Addgene_111459 Copy
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