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Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26830878
Comments: Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim WA. Cell. 2016 Feb 11;164(4):770-9. Epub 2016 Jan 28.
Proper citation: RRID:Addgene_79120 Copy
Species: Synthetic
Genetic Insert: LaG17 GFP nanobody synNotch TetRVP64
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26830878
Comments: Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim WA. Cell. 2016 Feb 11;164(4):770-9. Epub 2016 Jan 28.
Proper citation: RRID:Addgene_79128 Copy
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26830878
Comments: Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim WA. Cell. 2016 Feb 11;164(4):770-9. Epub 2016 Jan 28.
Proper citation: RRID:Addgene_79123 Copy
Species: Synthetic
Genetic Insert: antiCD19_synNotch_TetRVP64
Vector Backbone Description: Vector Backbone:pHR PGK; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26830878
Comments: Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim WA. Cell. 2016 Feb 11;164(4):770-9. Epub 2016 Jan 28.
Proper citation: RRID:Addgene_79126 Copy
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26830878
Comments: Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim WA. Cell. 2016 Feb 11;164(4):770-9. Epub 2016 Jan 28.
Proper citation: RRID:Addgene_79119 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79445 Copy
Genetic Insert: FLAG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pDEST26; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17846994
Proper citation: RRID:Addgene_79275 Copy
Species: Homo sapiens
Genetic Insert: GGA3
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:pGEX-4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11950392
Proper citation: RRID:Addgene_79436 Copy
Species: Homo sapiens
Genetic Insert: TCF7
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human TCF7 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79498 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human YAP1 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79503 Copy
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7649; Vector Backbone:pET28-MKH8SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please see http://www.thesgc.org/sites/default/files/toronto_vectors/pET28-MKH8SUMO.pdf for more information.
The pET28-MKH8SUMO vector was derived from expression plasmid pET28a-LIC (SGC). It is used for T7 promoter driven expression of recombinant proteins with the addition of an N-terminal fusion tag containing 8X His followed by a thrombin cleavage site, a SUMO, and a TEV cleavage site. Two lysines were encoded after the Met start site. Two stop codons are included in the vector at the downstream cloning site.
Insertion of DNA sequence into the cloning/expression region is performed using ClonTech In-Fusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI/BsaI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.The 15-bp primer extensions are compatible with those for pET28-MHL, such that the PCR inserts prepared for pET28-MHL can be directly used for cloning into pET28-MKH8SUMO.
Proper citation: RRID:Addgene_79526 Copy
Species: Homo sapiens
Genetic Insert: LXR Alpha
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for NR1H3 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79514 Copy
Vector Backbone Description: Backbone Marker:Chiu W; Backbone Size:4180; Vector Backbone:CaMV35S- sGFP(S65T)-NOS vector; Vector Types:Plant Expression, Reporter plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19219406
Proper citation: RRID:Addgene_79668 Copy
Genetic Insert: Pado Green
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27040905
Proper citation: RRID:Addgene_79663 Copy
Species: Homo sapiens
Genetic Insert: CSK22
Vector Backbone Description: Backbone Marker:Scott Gradia (Addgene plasmid # 78173); Vector Backbone:pET His10 TEV LIC cloning vector (2B-T-10); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30004690
Comments: This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
Proper citation: RRID:Addgene_79704 Copy
Genetic Insert: d2GFP-Zeb1 3'UTR
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27317311
Proper citation: RRID:Addgene_79601 Copy
Genetic Insert: d2GFP + miR-200 family targets
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27317311
Proper citation: RRID:Addgene_79602 Copy
Species: Homo sapiens
Genetic Insert: VRK1
Vector Backbone Description: Backbone Marker:Scott Gradia (Addgene plasmid # 78173); Vector Backbone:pET His10 TEV LIC cloning vector (2B-T-10); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30004690
Comments: This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
Proper citation: RRID:Addgene_79684 Copy
Species: Homo sapiens
Genetic Insert: FES
Vector Backbone Description: Backbone Marker:Scott Gradia (Addgene plasmid # 78173); Vector Backbone:pET His10 TEV LIC cloning vector (2B-T-10); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30004690
Comments: This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
Proper citation: RRID:Addgene_79686 Copy
Species: Mus musculus
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:4978; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27315482
Proper citation: RRID:Addgene_79671 Copy
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