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Species: Homo sapiens
Genetic Insert: Lipid binding Human TCR
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4798; Vector Backbone:pcDNA3.1(+)/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Lipid binding gamma delta TCR. 5' cloning site Esp31 (not destroyed). 3' cloning site Esp31 (not destroyed).
Proper citation: RRID:Addgene_128543 Copy
Species: Homo sapiens
Genetic Insert: Lipid binding Human TCR
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4784; Vector Backbone:pcDNA3.1(+)/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Lipid binding gamma delta TCR. 5' cloning site Esp31 (not destroyed). 3' cloning site Esp31 (not destroyed).
Addgene NGS found a single nucleotide deletion in the gamma chain, resulting in a frameshift and early termination of the translation, compared to the reference sequence provided by the Alcocer laboratory. The Alcocer laboratory has confirmed that this TCR receptor is functional and has been recently successfully used in their lab
Proper citation: RRID:Addgene_128542 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWE-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128656 Copy
Species: Homo sapiens
Genetic Insert: GATA2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11021798
Proper citation: RRID:Addgene_1287 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GW-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128684 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWE-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128683 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWE-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128682 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWE-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128680 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8316; Vector Backbone:pLVX-GWB-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128670 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWE-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128677 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWE-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128676 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8316; Vector Backbone:pLVX-GWB-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128675 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWE-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128679 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8048; Vector Backbone:pCT--CMV-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128666 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8048; Vector Backbone:pCT--CMV-IRES-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128665 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8316; Vector Backbone:pLVX-GWB-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128664 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8316; Vector Backbone:pLVX-GWB-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128663 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8626; Vector Backbone:pLVX-GWB-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Comments: An IS4 element was identified by Addgene QC that does not appear to be of functional concern but care should be taken by requesting scientists to ensure the expected functionality of the construct prior to use.
Proper citation: RRID:Addgene_128668 Copy
Species: Homo sapiens
Genetic Insert: PolB
Vector Backbone Description: Backbone Size:8626; Vector Backbone:pLVX-GWB-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25423885
Proper citation: RRID:Addgene_128667 Copy
Species: Homo sapiens
Genetic Insert: PARN sgRNA #2
Vector Backbone Description: Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29887379
Proper citation: RRID:Addgene_128760 Copy
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