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pTHY_P0022_T2c_BCD_0.75K
 
Resource Report
Resource Website 		RRID:Addgene_200054 BCD 0.75K Synthetic Ampicillin PMID:37253269 This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB. Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint. Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:36 0
H5_fold-0_Elsa
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_201825 H5_fold-0_Elsa Synthetic Ampicillin PMID:38177681 Backbone Size:5708; Vector Backbone:pET15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:31 1
sgRNA1 _1xCTS-ECFP-pA
 
Resource Report
Resource Website 		RRID:Addgene_200237 sgRNA1_1xCTS Synthetic Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint. Vector Backbone:p035_pause-HBG-CFP-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:36 0
sgRNA3 _1xCTS-ECFP-pA
 
Resource Report
Resource Website 		RRID:Addgene_200239 sgRNA3_1xCTS Synthetic Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint. Vector Backbone:p035_pause-HBG-CFP-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:31 0
sgRNA2_8xCTS-ECFP-pA
 
Resource Report
Resource Website 		RRID:Addgene_200244 sgRNA2_8xCTS Synthetic Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint. Vector Backbone:P2-ECFP-pA (Addgene #26280); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:37 0
mtsCOXVIIIx2-B-gTEMP/pcDNA3
 
Resource Report
Resource Website 		RRID:Addgene_199806 B-gTEMP Synthetic Ampicillin PMID:35792763 Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:30 0
sgRNA1 _8xCTS-ECFP-pA
 
Resource Report
Resource Website 		RRID:Addgene_200243 sgRNA1_8xCTS Synthetic Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint. Vector Backbone:P2-ECFP-pA (Addgene #26280); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:31 0
Myc-βarrestin2-LOV-Turbo_pCDNA3
 
Resource Report
Resource Website 		RRID:Addgene_199671 LOV-Turbo Synthetic Ampicillin PMID:37188954 Please visit https://www.biorxiv.org/content/10.1101/2023.03.09.531939v1 for bioRxiv preprint. Backbone Size:5366; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:32 0
pUCmini-iCAP-PHP.eC
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_201903 Synthetic construct isolate AAV-PHP.eC VP1 gene Synthetic Ampicillin PMID:37075114 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-29 01:17:35 1
pAS1 - pRig-3::GCaMP6f::unc-54
 
Resource Report
Resource Website 		RRID:Addgene_194970 GCaMP6f Synthetic Ampicillin Vector Backbone:Unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:35 0
pAAV-hSyn-JEDI-1P-Kv-WPRE
 
Resource Report
Resource Website 		RRID:Addgene_202612 JEDI-1P-Kv Synthetic Ampicillin PMID:37828037 Please visit https://doi.org/10.1101/2022.08.29.505018 for bioRxiv preprint. Backbone Size:4545; Vector Backbone:pAAV-hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-29 01:17:42 0
JAB2191
 
Resource Report
Resource Website 		RRID:Addgene_190355 proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt Synthetic Spectinomycin PMID:35951698 Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint. Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:17:35 0
JAB2192
 
Resource Report
Resource Website 		RRID:Addgene_190356 proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt Synthetic Spectinomycin PMID:35951698 Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint. Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:17:35 0
pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc
 
Resource Report
Resource Website 		RRID:Addgene_200123 Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase Synthetic Ampicillin PMID:36720982 Backbone Size:7138; Vector Backbone:pQCxIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:17:12 0
pQC.Flip-nluc(LgBiT1-8)CP[TEV]
 
Resource Report
Resource Website 		RRID:Addgene_200122 Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry Synthetic Ampicillin PMID:36720982 Backbone Size:7138; Vector Backbone:pQCxIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-29 01:17:14 0
pEM.A02L
 
Resource Report
Resource Website 		RRID:Addgene_198657 Adaptor Synthetic Kanamycin PMID:37058502 Cloning Method: Goldon Gate Assembly Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-29 01:17:13 0
pEM.A02R
 
Resource Report
Resource Website 		RRID:Addgene_198658 Adaptor Synthetic Kanamycin PMID:37058502 Cloning Method: Goldon Gate Assembly Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-29 01:17:16 0
pEGFP-C1-G2BRSCR
 
Resource Report
Resource Website 		RRID:Addgene_185345 scrambled gp78 G2BR sequence Synthetic Kanamycin PMID:34879065 Encodes a scrambled gp78 G2BR sequence: SKANDSRELQFRKMRLRAQRQVQELDKL Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin scrambled sequence 2026-08-29 01:17:12 0
pHEN2 F8-2
 
Resource Report
Resource Website 		RRID:Addgene_186033 VHH F8-2 Synthetic Ampicillin PMID:35007758 Backbone Size:4500; Vector Backbone:pHEN2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:17:13 0
pEM.A01L
 
Resource Report
Resource Website 		RRID:Addgene_198655 Adaptor Synthetic Kanamycin PMID:37058502 Cloning Method: Goldon Gate Assembly Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-29 01:17:15 0

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