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Species: Synthetic
Genetic Insert: BCD 0.75K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200054 Copy
Species: Synthetic
Genetic Insert: H5_fold-0_Elsa
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38177681
Proper citation: RRID:Addgene_201825 Copy
Species: Synthetic
Genetic Insert: sgRNA1_1xCTS
Vector Backbone Description: Vector Backbone:p035_pause-HBG-CFP-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200237 Copy
Species: Synthetic
Genetic Insert: sgRNA3_1xCTS
Vector Backbone Description: Vector Backbone:p035_pause-HBG-CFP-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200239 Copy
Species: Synthetic
Genetic Insert: sgRNA2_8xCTS
Vector Backbone Description: Vector Backbone:P2-ECFP-pA (Addgene #26280); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200244 Copy
Species: Synthetic
Genetic Insert: B-gTEMP
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35792763
Proper citation: RRID:Addgene_199806 Copy
Species: Synthetic
Genetic Insert: sgRNA1_8xCTS
Vector Backbone Description: Vector Backbone:P2-ECFP-pA (Addgene #26280); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200243 Copy
Species: Synthetic
Genetic Insert: LOV-Turbo
Vector Backbone Description: Backbone Size:5366; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37188954
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.03.09.531939v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_199671 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.eC VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075114
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_201903 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_194970 Copy
Species: Synthetic
Genetic Insert: JEDI-1P-Kv
Vector Backbone Description: Backbone Size:4545; Vector Backbone:pAAV-hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37828037
Comments: Please visit https://doi.org/10.1101/2022.08.29.505018 for bioRxiv preprint.
Proper citation: RRID:Addgene_202612 Copy
Species: Synthetic
Genetic Insert: proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt
Vector Backbone Description: Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951698
Comments: Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190355 Copy
Species: Synthetic
Genetic Insert: proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt
Vector Backbone Description: Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951698
Comments: Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190356 Copy
Species: Synthetic
Genetic Insert: Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
Vector Backbone Description: Backbone Size:7138; Vector Backbone:pQCxIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36720982
Proper citation: RRID:Addgene_200123 Copy
Species: Synthetic
Genetic Insert: Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
Vector Backbone Description: Backbone Size:7138; Vector Backbone:pQCxIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36720982
Proper citation: RRID:Addgene_200122 Copy
Species: Synthetic
Genetic Insert: Adaptor
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198657 Copy
Species: Synthetic
Genetic Insert: Adaptor
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198658 Copy
Species: Synthetic
Genetic Insert: scrambled gp78 G2BR sequence
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34879065
Comments: Encodes a scrambled gp78 G2BR sequence: SKANDSRELQFRKMRLRAQRQVQELDKL
Proper citation: RRID:Addgene_185345 Copy
Species: Synthetic
Genetic Insert: VHH F8-2
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pHEN2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35007758
Proper citation: RRID:Addgene_186033 Copy
Species: Synthetic
Genetic Insert: Adaptor
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198655 Copy
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