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Species: Homo sapiens
Genetic Insert: Mito
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mRaspberry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 598; Emission = 625
Proper citation: RRID:Addgene_55931 Copy
Species: Homo sapiens
Genetic Insert: ER
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mStrawberry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 574; Emission = 596
Proper citation: RRID:Addgene_55944 Copy
Species: Homo sapiens
Genetic Insert: TOMM20
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mPlum-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 590; Emission = 649
Proper citation: RRID:Addgene_56002 Copy
Species: Homo sapiens
Genetic Insert: VE-Cadherin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mPlum-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Excitation = 590; Emission = 649
Addgene QC NGS analysis identifies an I503T discrepancy in the insert sequence. It is unknown what, if any, impact this may have on plasmid function.
Proper citation: RRID:Addgene_56005 Copy
Species: Homo sapiens
Genetic Insert: Mito
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mKeima-Red; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 440; Emission = 620
Proper citation: RRID:Addgene_56018 Copy
Species: Homo sapiens
Genetic Insert: ER
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mPlum; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 590; Emission = 649
Proper citation: RRID:Addgene_55966 Copy
Species: Synthetic
Genetic Insert: nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
Vector Backbone Description: Backbone Size:6200; Vector Backbone:phage; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24402470
Proper citation: RRID:Addgene_52985 Copy
Vector Backbone Description: Backbone Size:5935; Vector Backbone:pMos3xP3DsRED; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20456509
Comments: attP site is gtagtgccccaactggggtaacctttgagttctctcagttgggggcgtag
Proper citation: RRID:Addgene_52904 Copy
Species: west nile virus
Genetic Insert: prM-E
Vector Backbone Description: Vector Backbone:pPSClo; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52882 Copy
Genetic Insert: lck-GCaMP6f
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4
Vector Backbone Description: Backbone Marker:David Strutt (Brittle et al., 2010); Vector Backbone:pAct5-FRT-stop-FRT-SV40polyA attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25605786
Proper citation: RRID:Addgene_52889 Copy
Species: Homo sapiens
Genetic Insert: shRNA targeting mitogen-activated protein kinase p38
Vector Backbone Description: Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24670723
Proper citation: RRID:Addgene_52920 Copy
Species: Mus musculus
Genetic Insert: mP2X4-pHluorin123
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24935743
Proper citation: RRID:Addgene_52926 Copy
Species: Aedes aegypti
Genetic Insert: polyubiquitin promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4747; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20456509
Proper citation: RRID:Addgene_52891 Copy
Species: E.coli
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21189298
Comments: This strain is a precursor to HL 3262 (identical strain, except KanR cassette on chromosome allows for selection).
Proper citation: RRID:Addgene_52936 Copy
Species: E.coli
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None
Proper citation: RRID:Addgene_52951 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:10130; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25075903
Comments: Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
Proper citation: RRID:Addgene_52962 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25075903
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9.
Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
Proper citation: RRID:Addgene_52963 Copy
Species: Drosophila melanogaster
Genetic Insert: SIK3
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21565616
Proper citation: RRID:Addgene_52972 Copy
Species: Drosophila melanogaster
Genetic Insert: SIK3
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21565616
Proper citation: RRID:Addgene_52976 Copy
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