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Species: Mus musculus
Genetic Insert: Kruppel-like factor 4 (gut)
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16904174
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Mouse Klf4 includes G372A, A459C, G527C, G633C, G1021C, C1305G and T1368C mutations.
All of substitutions can be found in EST database of NCBI. So we believe that these differences are caused by SNPs.
As we have shown in the previous our papers, these changes do not affect biological functions of the transcription factors during reprogramming.
Proper citation: RRID:Addgene_13370 Copy
Species: Mus musculus
Genetic Insert: undifferentiated embryonic cell transcription factor 1
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16904174
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_13369 Copy
Species: Mus musculus
Genetic Insert: SRY-box containing gene 2
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16904174
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag. If
this cDNA is used in a non-tagged construct, a functional protein (missing the first 3 amino acids--MYN) is produced as confirmed by co-immunoprecipitation, reporter assay, and
other experiments.
Proper citation: RRID:Addgene_13367 Copy
Species: Mus musculus
Genetic Insert: Zinc finger protein 42
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16904174
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_13368 Copy
Species: Mus musculus
Genetic Insert: Gsdmd-NT
Vector Backbone Description: Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27383986
Proper citation: RRID:Addgene_133892 Copy
Species: Mus musculus
Genetic Insert: Gsdmd-NT
Vector Backbone Description: Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27383986
Proper citation: RRID:Addgene_133887 Copy
Species: Mus musculus
Genetic Insert: SPOP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30244836
Proper citation: RRID:Addgene_169720 Copy
Species: Mus musculus
Genetic Insert: Super ecliptic pHluorin (SEP)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33689678
Proper citation: RRID:Addgene_169442 Copy
Species: Mus musculus
Genetic Insert: Actb
Vector Backbone Description: Backbone Size:6061; Vector Backbone:pCAFNF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161760
Proper citation: RRID:Addgene_169788 Copy
Species: Mus musculus
Genetic Insert: safe harbor sgRNA
Vector Backbone Description: Backbone Size:8907; Vector Backbone:pMCB320; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157015
Proper citation: RRID:Addgene_169836 Copy
Species: Mus musculus
Genetic Insert: Grin1
Vector Backbone Description: Vector Backbone:gRNA backbone; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161760
Proper citation: RRID:Addgene_169790 Copy
Species: Mus musculus
Genetic Insert: Grin1
Vector Backbone Description: Vector Backbone:gRNA backbone; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161760
Proper citation: RRID:Addgene_169791 Copy
Species: Mus musculus
Genetic Insert: Circular 200,100 guide RNA
Vector Backbone Description: Backbone Marker:Addgene #78601; Backbone Size:5700; Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35145312
Proper citation: RRID:Addgene_170123 Copy
Species: Mus musculus
Genetic Insert: nuclear export factor 1, B6 allele
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7002; Vector Backbone:Modified pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170266 Copy
Species: Mus musculus
Genetic Insert: Partial sense strand of the mouse Tbxas1 gene for in situ hybridization
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4000; Vector Backbone:pCR-BluntII-TOPO; Vector Types:For IVF; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34035084
Proper citation: RRID:Addgene_170307 Copy
Species: Mus musculus
Genetic Insert: Partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4000; Vector Backbone:pCR-BluntII-TOPO; Vector Types:For IVF; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34035084
Proper citation: RRID:Addgene_170308 Copy
Species: Mus musculus
Genetic Insert: GluA2
Vector Backbone Description: Backbone Size:9370; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514725
Proper citation: RRID:Addgene_170453 Copy
Species: Mus musculus
Genetic Insert: FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pBabe-tetoff-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279442
Comments: The plasmid contains the Component2 - T2A P2A - Component1 fragment insert in a different backbone. Please refer to Addgene NGS result for full backbone sequence.
Proper citation: RRID:Addgene_170488 Copy
Species: Mus musculus
Genetic Insert: Cavin4
Vector Backbone Description: Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33904409
Proper citation: RRID:Addgene_170528 Copy
Species: Mus musculus
Genetic Insert: Chantico
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Comments: The mouse Chantico lncRNA promoter was inserted in the pUC19 cloning vector.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.23.474040v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170659 Copy
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