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Species: Homo sapiens
Genetic Insert: Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28346450
Proper citation: RRID:Addgene_89155 Copy
Species: HIV
Genetic Insert: IN
Vector Backbone Description: Backbone Marker:Warner Greene; Backbone Size:5000; Vector Backbone:pEGFP-PK; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:13130095
Comments: Generates alternate linker (Gly-Ser-Ser-Gly) in between PK and IN to compare to results of pPS2986. Localizes to cytoplasm.
Proper citation: RRID:Addgene_8929 Copy
Species: HIV
Genetic Insert: IN
Vector Backbone Description: Backbone Marker:Warner Greene; Backbone Size:5000; Vector Backbone:pEGFP-PK; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:13130095
Comments: Generates pEGFP-PK-codon optimized IN fusion. Localizes to cytoplasm.
Proper citation: RRID:Addgene_8928 Copy
Species: Rattus norvegicus
Genetic Insert: rAPOBEC1-nCas9 (D10A)-UGI-NLS
Vector Backbone Description: Backbone Size:5379; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28244995
Proper citation: RRID:Addgene_89508 Copy
Species: Synthetic
Genetic Insert: Asparaginyl endopeptidase from Oldenlandia affinis
Vector Backbone Description: Backbone Size:4540; Vector Backbone:pHYRSF53; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28369872
Proper citation: RRID:Addgene_89482 Copy
Species: synthetic construct
Genetic Insert: glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5240; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_89593 Copy
Species: synthetic construct
Genetic Insert: glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5240; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_89595 Copy
Species: Homo sapiens
Genetic Insert: Optop38i dark-state mutant
Vector Backbone Description: Backbone Marker:Derived by our lab using pExFP-C1 and pGL3; Backbone Size:5673; Vector Backbone:pLuc-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.
Proper citation: RRID:Addgene_89748 Copy
Species: Synthetic
Genetic Insert: OptoJNKi
Vector Backbone Description: Backbone Marker:Derived by our lab using pExFP-C1 and pGL3; Backbone Size:5673; Vector Backbone:pLuc-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: Please see
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017 in press.
Proper citation: RRID:Addgene_89744 Copy
Species: Synthetic
Genetic Insert: NLS-OptoJNKi dark-state mutant
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression, Fluorescent protein; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.
Proper citation: RRID:Addgene_89743 Copy
Species: Synthetic
Genetic Insert: NLS-OptoJNKi lit-state mutant
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression, Fluorescent protein; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.
Proper citation: RRID:Addgene_89742 Copy
Species: Synthetic
Genetic Insert: NLS-OptoJNKi
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression, Fluorescent protein; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28497795
Comments: For further information please see:
Melero-Fernandez de Mera RM*, Li LL*, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ (2017) A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat. Commun. 8, 15017 doi: 10.1038/ncomms15017.
Proper citation: RRID:Addgene_89739 Copy
Genetic Insert: SMT3_GLP_H6_GFP_TRNGL
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3669; Vector Backbone:pRSF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28369872
Proper citation: RRID:Addgene_89699 Copy
Genetic Insert: SMT3_GLP_H6_GFP_TRNAL
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3669; Vector Backbone:pRSF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28369872
Proper citation: RRID:Addgene_89701 Copy
Species: Methanococcus voltae
Genetic Insert: AglC
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5310; Vector Backbone:pET24a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28935101
Proper citation: RRID:Addgene_89726 Copy
Species: Campylobacter jejuni
Genetic Insert: PseH
Vector Backbone Description: Backbone Marker:EMB Biosciences; Backbone Size:5400; Vector Backbone:pET30b (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28935101
Proper citation: RRID:Addgene_89725 Copy
Species: Campylobacter jejuni
Genetic Insert: PseC
Vector Backbone Description: Backbone Marker:EMB Biosciences; Backbone Size:5400; Vector Backbone:pET30b (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28935101
Proper citation: RRID:Addgene_89724 Copy
Species: Campylobacter jejuni
Genetic Insert: PseB
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5310; Vector Backbone:pET24a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28935101
Proper citation: RRID:Addgene_89723 Copy
Species: Methanococcus voltae
Genetic Insert: AglK
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5310; Vector Backbone:pET24a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28935101
Proper citation: RRID:Addgene_89714 Copy
Species: Synthetic
Genetic Insert: wm_ruleB
Vector Backbone Description: Backbone Marker:Eurofins; Vector Backbone:pEX-K4; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28336611
Proper citation: RRID:Addgene_89675 Copy
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