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Species: Mus musculus
Genetic Insert: 18S
Vector Backbone Description: Backbone Marker:promega; Backbone Size:2000; Vector Backbone:pCMV; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22718970
Proper citation: RRID:Addgene_51730 Copy
Species: Mus musculus
Genetic Insert: Jnk interacting protein 1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9235893
Proper citation: RRID:Addgene_51699 Copy
Species: Mus musculus
Genetic Insert: SHPK
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:peGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21348809
Proper citation: RRID:Addgene_51748 Copy
Species: Mus musculus
Genetic Insert: SHPK
Vector Backbone Description: Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21348809
Proper citation: RRID:Addgene_51747 Copy
Species: Mus musculus
Genetic Insert: Jnk interacting protein 1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9235893
Proper citation: RRID:Addgene_51766 Copy
Species: Mus musculus
Genetic Insert: Fbxl3
Vector Backbone Description: Vector Backbone:pfastbac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23452855
Proper citation: RRID:Addgene_51894 Copy
Species: Mus musculus
Genetic Insert: Fbxl21
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pTRE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23452855
Proper citation: RRID:Addgene_51890 Copy
Species: Mus musculus
Genetic Insert: DN JNK1
Vector Backbone Description: Backbone Size:8800; Vector Backbone:pACCMVpLpA (pLpA); Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14517246
Proper citation: RRID:Addgene_51942 Copy
Species: Mus musculus
Genetic Insert: presynaptic mGRASP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:paavCAG-Jx; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22138823
Proper citation: RRID:Addgene_51900 Copy
Species: Mus musculus
Genetic Insert: presynaptic mGRASP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:paavCAG; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22138823
Proper citation: RRID:Addgene_51902 Copy
Species: Mus musculus
Genetic Insert: presynaptic mGRASP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:paavCAG-Jx; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24412418
Comments: Please note that there may be a few sequence discrepancies between Addgene's NGS results for this plasmid and the reference map provided by the depositing lab. These changes are in the backbone region outside of the viral cassette and should not affect function.
Proper citation: RRID:Addgene_51903 Copy
Species: Mus musculus
Genetic Insert: mFbxl3
Vector Backbone Description: Backbone Marker:sigma; Vector Backbone:pCMV10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23452855
Proper citation: RRID:Addgene_51888 Copy
Species: Mus musculus
Genetic Insert: GATA6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11356841
Comments: SalI site is 120bp 5' of ATG and XbaI site comes from bluescript.
Proper citation: RRID:Addgene_51929 Copy
Species: Mus musculus
Genetic Insert: GATA4 reverse orientation
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11356841
Comments: 1.7 kb mouse GATA4 EcoRI fragment, 80 bp of 5'UTR
Proper citation: RRID:Addgene_51928 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24305048
Comments: let-7 sequence for nucleotides 2-15 is CAACCTACTACCTCA.
Proper citation: RRID:Addgene_52037 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24305048
Proper citation: RRID:Addgene_52034 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24305048
Proper citation: RRID:Addgene_52038 Copy
Species: Mus musculus
Genetic Insert: Ngn2
Vector Backbone Description: Backbone Size:7745; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764284
Proper citation: RRID:Addgene_52047 Copy
Species: Mus musculus
Genetic Insert: T cell receptor alpha chain
Vector Backbone Description: Backbone Marker:Dario Vignali, Addgene plasmid 52107; Vector Backbone:pMIG II; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17406263
Comments: The cDNAs encoding the TCRa (lacking the stop codon) and TCRb chains were linked via a 2A peptide into a single open reading frame by recombinant PCR and cloned into a mouse stem cell virus (MSCV)-based retroviral vector containing an IRES-GFP cassette to facilitate the identification of marked cells.
The 2A peptide remains attached to the TCRa carboxy terminus and can be used to verify the complete separation of the two chains. Inclusion of a Gly-Ser-Gly (GSG) spacer between TCRa and the 2A peptide ensures complete ‘cleavage’. We have not seen any deleterious effects of the 2A peptide on the function of any proteins examined thus far.
Proper citation: RRID:Addgene_52111 Copy
Species: Mus musculus
Genetic Insert: T cell receptor alpha chain
Vector Backbone Description: Backbone Marker:Dario Vignali, Addgene plasmid 52107; Vector Backbone:pMIG II; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17406263
Comments: The cDNAs encoding the TCRa (lacking the stop codon) and TCRb chains were linked via a 2A peptide into a single open reading frame by recombinant PCR and cloned into a mouse stem cell virus (MSCV)-based retroviral vector containing an IRES-GFP cassette to facilitate the identification of marked cells.
The 2A peptide remains attached to the TCRa carboxy terminus and can be used to verify the complete separation of the two chains. Inclusion of a Gly-Ser-Gly (GSG) spacer between TCRa and the 2A peptide ensures complete ‘cleavage’. We have not seen any deleterious effects of the 2A peptide on the function of any proteins examined thus far.
Proper citation: RRID:Addgene_52112 Copy
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