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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pMCB320-sgNC.SPA
 
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RRID:Addgene_169834 safe harbor sgRNA Mus musculus Ampicillin PMID:33157015 Backbone Size:8907; Vector Backbone:pMCB320; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:02 0
XP12 Xbra
 
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RRID:Addgene_16978 Xbra Xenopus laevis Ampicillin Insert: full length Xbra from Jim Smith. Antisense: linearize with EcoRV, transcribe with T7. Plasmid History: Constructed by Jan Christian. Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:psp73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:02 0
LentiCRISPRv2-KLF7-1
 
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RRID:Addgene_169797 guide RNA for KLF7 neighbouring region Homo sapiens Ampicillin PMID:30733539 Backbone Marker:Addgene Plasmid #52961; Backbone Size:12993; Vector Backbone:LentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:02 0
pBS-ACTB-C200-GFPfr1
 
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1+ mentions
RRID:Addgene_169798 Donor sequence to ACTB gene for HR Homo sapiens Ampicillin PMID:30733539 Backbone Marker:Stratagene; Backbone Size:2959; Vector Backbone:pBlueScript II SK (+); Vector Types:HR donor vector for human ACTB gene.; Bacterial Resistance:Ampicillin Partial sequence of GFP is inserted before the stop codon of ACTB gene. 2026-08-15 01:10:02 3
pAAV.hSynapsin1.OxLight1
 
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1+ mentions
RRID:Addgene_169792 OxLight1 Synthetic Ampicillin PMID:35145320 Backbone Marker:Viral Vector Facility - University of Zurich; Backbone Size:4438; Vector Backbone:pAAV.hSynapsin1; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:10:02 1
LentiCRISPRv2-scr
 
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1+ mentions
RRID:Addgene_169795 Scrambled guide RNA Ampicillin PMID:30733539 Backbone Marker:Addgene Plasmid #52961; Backbone Size:12993; Vector Backbone:LentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:02 5
pFnCas12a_NT
 
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RRID:Addgene_169838 FnCas12a Francisella tularensis subsp. novicida Chloramphenicol PMID:33538626 Backbone Size:4447; Vector Backbone:pNW33n; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:02 0
pPIDCB2
 
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RRID:Addgene_169900 Ampicillin PMID:32917762 Use AflII and PstI restriction sites for cloning. This plasmid is identical to pPIDNB2 except it has replaced the mNeonGreen sequence in pPIDNB2, which requires a paid Allele Biotech license, with mClover3, which doesn't require a paid license. Backbone Size:6529; Vector Backbone:pPIDCB2; Vector Types:Mammalian Expression, Avian expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:03 0
Negative feedback (NF) plasmid
 
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RRID:Addgene_169747 tetR-P2A-eGFP Synthetic Ampicillin PMID:34226556 Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid. Backbone Marker:Invitrogen; Backbone Size:6113; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:10:02 0
pPIDNB2
 
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RRID:Addgene_169902 Ampicillin PMID:32917762 Use AflII and PstI restriction sites for cloning. This plasmid contains mNeonGreen, which requires a paid license. See pPIDCB2 for an alternative plasmid, which has replaced the mNeongreen with mClover3 and does not require a paid license. Backbone Size:6916; Vector Backbone:pPIDNB2; Vector Types:Mammalian Expression, Avian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:02 0
U6-U57A
 
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RRID:Addgene_169927 U6 U57A Saccharomyces cerevisiae Ampicillin PMID:33547186 Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone. Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:03 0
U6-U57C
 
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RRID:Addgene_169928 U6 U57C Saccharomyces cerevisiae Ampicillin PMID:33547186 Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone. Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:03 0
pET-17b_K490clm_E215C
 
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RRID:Addgene_169995 kinesin family member 5B, isoform CRA_a Homo sapiens Ampicillin PMID:30770448 Backbone Marker:Novagene; Backbone Size:3306; Vector Backbone:pET-17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C7S, C65A, C168A, C174S, E215C, C294A, C330S, C421A 2026-08-15 01:10:04 0
pET-17b_Unc104-KIF5B-HALO
 
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RRID:Addgene_169996 Kinesin-like protein Unc-104 fused with KIF5B Homo sapiens Ampicillin PMID:32203385 Backbone Marker:Novagene; Backbone Size:3306; Vector Backbone:pET-17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:04 0
pKB232
 
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RRID:Addgene_170019 mNeonGreen Branchiostoma lanceolatum Ampicillin Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009). Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:10:04 0
sgRNA.SFFV.tBFP
 
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1+ mentions
RRID:Addgene_169940 Ampicillin Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:10:03 1
pKB226
 
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RRID:Addgene_170016 mNeonGreen Branchiostoma lanceolatum Ampicillin Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009). Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:10:04 0
Bison sgRNA library
 
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1+ mentions
RRID:Addgene_169942 guide RNAs targeting E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes Ampicillin PMID:32494016 Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:10:03 1
pKB224
 
Resource Report
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RRID:Addgene_170014 mNeonGreen Branchiostoma lanceolatum Ampicillin Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009). Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:10:04 0
pKB222
 
Resource Report
Resource Website
RRID:Addgene_170012 mNeonGreen Branchiostoma lanceolatum Ampicillin Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009). Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint. Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:10:04 0

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