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Genetic Insert: Tre-C04
Vector Backbone Description: Backbone Size:3712; Vector Backbone:pTKEI-Dest; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27470466
Proper citation: RRID:Addgene_79754 Copy
Species: Escherichia coli (EcNR1)
Genetic Insert: Lambda Red recombinase beta subunit
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26496947
Proper citation: RRID:Addgene_79968 Copy
Species: Synthetic
Genetic Insert: human codon-optimized Vika sequence, NLS tagged
Vector Backbone Description: Vector Backbone:pNPK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23143104
Proper citation: RRID:Addgene_79969 Copy
Species: Synthetic
Genetic Insert: miRFP709
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3999; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27539380
Proper citation: RRID:Addgene_79986 Copy
Species: Danio rerio
Genetic Insert: HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Vector Backbone Description: Backbone Size:2994; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28402863
Proper citation: RRID:Addgene_79887 Copy
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26456817
Comments: This plasmid is derived from eSpCas9(1.1) (Plasmid #71814) from Feng Zhang's lab. The 3x FLAG sequence was removed to prevent interference of the tag in western blot and immunoprecipitation applications.
The gRNA sequence is derived from gRNA_AAVS1-T2 (Plasmid #41818) from George Church's lab.
Proper citation: RRID:Addgene_79888 Copy
Species: Danio rerio
Genetic Insert: Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Vector Backbone Description: Backbone Size:6903; Vector Backbone:pMT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28402863
Proper citation: RRID:Addgene_79885 Copy
Species: Streptococcus pyogenes
Genetic Insert: sgRNA RR1
Vector Backbone Description: Backbone Size:6673; Vector Backbone:pDG1731; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27238023
Comments: Please note that mutation T23S in the BSU homology region was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_79875 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pCB301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17720752
Proper citation: RRID:Addgene_80083 Copy
Species: Homo sapiens
Genetic Insert: hGeminin (1/110)
Vector Backbone Description: Backbone Marker:Dr. Hiroyuki Miyoshi (RIKEN, Tsukuba, Japan); Vector Backbone:pCSII-EF-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27539380
Proper citation: RRID:Addgene_80006 Copy
Genetic Insert: Cas1
Vector Backbone Description: Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27284167
Proper citation: RRID:Addgene_80089 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_80036 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_80035 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_80034 Copy
Species: Vesicular Stomatitis Virus
Genetic Insert: VSV-G-Myc
Vector Backbone Description: Vector Backbone:na; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80054 Copy
Species: Vesicular Stomatitis Virus
Genetic Insert: VSV-G-Myc(P127D)
Vector Backbone Description: Vector Backbone:na; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80055 Copy
Species: Other
Genetic Insert: OsTIR1
Vector Backbone Description: Backbone Size:5213; Vector Backbone:pBabe; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29804665
Comments: The osTIR1-9Myc was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
Proper citation: RRID:Addgene_80074 Copy
Genetic Insert: Cas1
Vector Backbone Description: Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27284167
Proper citation: RRID:Addgene_80102 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27147599
Comments: Open state: NO stop codon immediately follows insert
Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
Proper citation: RRID:Addgene_81742 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27147599
Comments: Open state: NO stop codon immediately follows insert
Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
Proper citation: RRID:Addgene_81736 Copy
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