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Species: Homo sapiens
Genetic Insert: PKD1 RNAi
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12505989
Comments: Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCATG CTGTGGGGGC TGGTACTTCA AGAGAGTACC AGCCCCCACA GCATTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAATGCTG TGGGGGCTGG TACTCTCTTG AAGTACCAGC CCCCACAGCA TGGG-3'.
Proper citation: RRID:Addgene_10815 Copy
Species: Synthetic
Genetic Insert: Blue fluorescent protein (BFP2)
Vector Backbone Description: Backbone Marker:Brett Stringer; Vector Backbone:pF CAG luc IRES neo (Addgene plasmid # 98294); Vector Types:Mammalian Expression, Lentiviral, Fluorescent protein; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30894629
Proper citation: RRID:Addgene_108175 Copy
Vector Backbone Description: Backbone Marker:Jörg Kudla; Backbone Size:14556; Vector Backbone:UBQ10:sXVE:GFPc:Kan; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29451720
Proper citation: RRID:Addgene_108178 Copy
Vector Backbone Description: Backbone Marker:Jörg Kudla; Backbone Size:14452; Vector Backbone:UBQ10:sXVE:GFPc:Kan; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29451720
Proper citation: RRID:Addgene_108177 Copy
Species: B. microti
Genetic Insert: BMR1_02g00615
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108029 Copy
Species: B. microti
Genetic Insert: BMR1_04g05295
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108027 Copy
Species: B. microti
Genetic Insert: BMR1_03g04550
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108026 Copy
Species: B. microti
Genetic Insert: BMR1_03g04855
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108024 Copy
Species: B. microti
Genetic Insert: BMR1_03g01540
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108043 Copy
Species: B. microti
Genetic Insert: BmR1_04g07470
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108037 Copy
Species: B. microti
Genetic Insert: BMR1_04g09435
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108034 Copy
Species: Synthetic
Genetic Insert: sgRNA targeting GGCGGCGGCGGCGGCGGCGG
Vector Backbone Description: Backbone Marker:Addgene 84477; Vector Backbone:pgRNA-modified; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29456084
Comments: contain a CMV-Puro-T2A-mCherry cassette allowing for FACS isolation or puromycin selection.
Proper citation: RRID:Addgene_108248 Copy
Species: Synthetic
Genetic Insert: dCas9-dead Tet1CD-HA-P2A-BFP
Vector Backbone Description: Backbone Size:9216; Vector Backbone:Fuw; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29456084
Proper citation: RRID:Addgene_108246 Copy
Species: Synthetic
Genetic Insert: dCas9-Tet1CD-HA-P2A-BFP
Vector Backbone Description: Backbone Size:9216; Vector Backbone:Fuw; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29456084
Proper citation: RRID:Addgene_108245 Copy
Species: Synthetic
Genetic Insert: Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
Vector Backbone Description: Backbone Size:6438; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28946740
Proper citation: RRID:Addgene_108244 Copy
Species: Synthetic
Genetic Insert: Membrane bound transcription activator CadC fusing with anti-RNase A VHH antibody
Vector Backbone Description: Backbone Size:6438; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28946740
Proper citation: RRID:Addgene_108243 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5313; Vector Backbone:pIRESneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260970
Comments: A small insertion in the 5'UTR should not affect the expressed peptide.
Proper citation: RRID:Addgene_10821 Copy
Vector Backbone Description: Backbone Marker:Jörg Kudla; Backbone Size:16025; Vector Backbone:UBQ10:sXVE:GFPc:Kan; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29451720
Proper citation: RRID:Addgene_108260 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30478458
Comments: The three new “Marionette” strains contain the Marionette cluster, which expresses 12 inducible transcription factors, from the genome. Plasmids pAJM.711, pAJM.712, pAJM.713, pAJM.714, pAJM.715, pAJM.717, pAJM.716, pAJM.718, pAJM.719, pAJM.1459, pAJM.721 are reporters for those 12 transcription factors. For examples: TetR is being expressed from the genome of the Marionette strains, and pAJM.717 has a promoter that is repressed by TetR (PTet*) driving YFP. pAJM.717 should not produce YFP, until the cell is exposed to anhydrotetracycline (aTc). Other plasmids from our laboratory encode both the reporter and the transcription factor, and should be used in a regular (non-Marionette) strain. For example: pAJM.011 expresses TetR and contains a PTet*-YFP reporter module.
Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108254 Copy
Species: Synthetic
Genetic Insert: S. solfataricus PCNA1
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5330; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108197 Copy
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