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Species: Hepatitis C Virus
Genetic Insert: NS4A
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24130685
Comments: For protein expression, grow in Rosetta cells.
25 degree induction for 14-15 hours with 0.5 mM IPTG
Proper citation: RRID:Addgene_61696 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61737 Copy
Species: E. coli
Genetic Insert: nptII gene
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCGEN (pCAMBIA-0380 backbone with nptII gene under Neurospora crassa gpd promoter).
NptII (neomycin phosphotransferase II) gene from pCGEN was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-G418-YR.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and nptII sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Please note that the Addgene verified sequence identified a transposase in the backbone (7145-8353 bp).
Proper citation: RRID:Addgene_61767 Copy
Species: MS2 virus
Genetic Insert: MS2 coat protein fused to eGFP
Vector Backbone Description: Backbone Size:4781; Vector Backbone:Lentiviral expression vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25271374
Proper citation: RRID:Addgene_61764 Copy
Species: Synthetic
Genetic Insert: Hygromycin
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCHYG. For more information, see: Motteram et al, 2009. Molecular Plant-Microbe Interactions, 22, 790-799. https://www.ncbi.nlm.nih.gov/pubmed/19522561.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and hygromycin sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication (Sidhu et al., 2015) for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Proper citation: RRID:Addgene_61765 Copy
Species: Mus musculus
Genetic Insert: mouse Zeb2 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Proper citation: RRID:Addgene_61771 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7988; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61702 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61703 Copy
Vector Backbone Description: Backbone Size:11775; Vector Backbone:pEarleyGate-202; Vector Types:Plant Expression, BiFC assay; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61705 Copy
Species: Homo sapiens
Genetic Insert: OMA1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_61719 Copy
Species: túngara frog (Engystomops pustulosus)
Genetic Insert: Ranaspumin-2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET-28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19527658
Proper citation: RRID:Addgene_61686 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Exactly the same as pEYFP-N1 but with AgeI-BsrGI swapped for venus fragment containing the point mutations F46L/F64L/V68L/M153T/V163A/S175G compared to EYFP
Proper citation: RRID:Addgene_61854 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61887 Copy
Species: Kluyveromyces lactis
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21768125
Proper citation: RRID:Addgene_61923 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61883 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61885 Copy
Species: Homo sapiens
Genetic Insert: hUCH37 (ISF1)
Vector Backbone Description: Backbone Marker:Invotrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25702872
Proper citation: RRID:Addgene_61929 Copy
Species: Thermoplasma volcanium
Genetic Insert: TvoVMAN11 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61813 Copy
Species: Homo sapiens
Genetic Insert: cyclin A
Vector Backbone Description: Backbone Marker:Amersham; Vector Backbone:pGEX 6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61844 Copy
Species: E. coli
Genetic Insert: lacZ alpha
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_61972 Copy
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