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Species: Homo sapiens
Genetic Insert: ERK5
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Proper citation: RRID:Addgene_53171 Copy
Species: Mus musculus
Genetic Insert: CMYC
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The mutations found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53178 Copy
Genetic Insert: Tshld-GFP
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4470; Vector Backbone:pDONR 201; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24615791
Comments: The Tshld system allows users to fine-tune the concentration of a protein of interest in mammalian cells by titrating the concentration of rapamycin or FK506. Unlike other post-translational techniques, the Tshld system releases its protein of interest in its native form, so it is highly attractive to be used in investigations of proteins involved in multimeric protein complexes.
Proper citation: RRID:Addgene_53211 Copy
Species: Homo sapiens
Genetic Insert: ERK2
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The sequence discrepancies found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53174 Copy
Genetic Insert: pCSaE500
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pCDFDuet-1; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23169002
Proper citation: RRID:Addgene_53182 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Marker:Rinehart lab, Addgene plasmid 52053; Vector Backbone:pCRT7; Vector Types:Bacterial Expression, phosphoprotein synthesis; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_53225 Copy
Vector Backbone Description: Backbone Size:3504; Vector Backbone:pzDonor; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25122746
Proper citation: RRID:Addgene_53189 Copy
Vector Backbone Description: Backbone Size:3515; Vector Backbone:pzDonor; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25122746
Proper citation: RRID:Addgene_53188 Copy
Species: Other
Genetic Insert: URA3-KanMX6
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23878277
Proper citation: RRID:Addgene_53194 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Proper citation: RRID:Addgene_53195 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53233 Copy
Species: Synthetic
Genetic Insert: monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043132
Proper citation: RRID:Addgene_53234 Copy
Species: Homo sapiens
Genetic Insert: Par4
Vector Backbone Description: Backbone Marker:Mark Davis lab; Vector Backbone:pBJ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_53231 Copy
Species: Homo sapiens
Genetic Insert: pMyc4ElbLuc
Vector Backbone Description: Vector Backbone:pG5ElbLuc; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8386367
Proper citation: RRID:Addgene_53246 Copy
Species: Synthetic
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Lutz and Bujard 1997; Backbone Size:4000; Vector Backbone:pzs*11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23277573
Proper citation: RRID:Addgene_53241 Copy
Species: Homo sapiens
Genetic Insert: RanGAP1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22350878
Proper citation: RRID:Addgene_53139 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyE
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9476; Vector Backbone:pAC-LYC; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8798688
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53273 Copy
Genetic Insert: HA tag
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pCG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2302733
Proper citation: RRID:Addgene_53395 Copy
Species: Synechococcus PCC7942
Genetic Insert: pds
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:8106; Vector Backbone:pAC-PHYT; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53316 Copy
Species: Erwinia herbicola (Pantoea agglomerans) Eho10
Genetic Insert: crtE, crtY, crtI
Vector Backbone Description: Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53282 Copy
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