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Species: Mus musculus
Genetic Insert: Synaptophysin-pHluorin
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27429220
Proper citation: RRID:Addgene_168510 Copy
Species: Mus musculus
Genetic Insert: dnWnt-1 myc CS2+
Vector Backbone Description: Backbone Size:5010; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Transcription: For sense, linearize with Asp718 (BMB) and transcribe with.
SP6. PCR amplified from myc tagged mouse Wnt-1 (McMahon and Moon). Also.
referred to as int-1 spnfc or int-1 spnfcD in notebook #3. The Wnt-1 gene.
is truncated acter the SPNFC peptide sequence.
Notes for Use:
Constructed by J. Brown.
Proper citation: RRID:Addgene_16859 Copy
Species: Mus musculus
Genetic Insert: Wnt1
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, in the Stratagene vector, pcDNA3. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.
Species of Sequence Origin: mouse.
Notes for Use: Uncharacterized.
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished version was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16854 Copy
Species: Mus musculus
Genetic Insert: antisense Wnt-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCD-betaG-PS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Description: Antisense orientation of full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, cloned into the SV40 expression vector pCD-PS, but providing Xenopus beta globin 5' and 3' UTRs.
Species of Sequence Origin: mouse.
Notes for Use: Uncharacterized.
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16852 Copy
Species: Mus musculus
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mouse Targeting, Cre/Lox, dre / rox; Bacterial Resistance:Ampicillin
Comments: NGS sequencing during the QC process discovered mutations in the TagRFP-T-drePBD* sequence. Although these disrupt the drePBD* expression, this plasmid was deposited as is because the depositors had already generated a mouse line with the construct. The AU1 epitope tagged Id1 in that mouse line (with or without dre / rox recombination) should still be functional.
Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Proper citation: RRID:Addgene_168770 Copy
Species: Mus musculus
Genetic Insert: Hrasls3
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-IPU-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_168771 Copy
Species: Mus musculus
Genetic Insert: sgRNA targeting Zmat3, mCherry, Puromycin resistance
Vector Backbone Description: Backbone Size:8907; Vector Backbone:pMCB320; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157015
Proper citation: RRID:Addgene_169026 Copy
Species: Mus musculus
Genetic Insert: sgRNA targeting Zmat3, mCherry, Puromycin resistance
Vector Backbone Description: Backbone Size:8907; Vector Backbone:pMCB320; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157015
Proper citation: RRID:Addgene_169024 Copy
Species: Mus musculus
Genetic Insert: RiboL1-jGCaMP8m
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_169248 Copy
Species: Mus musculus
Genetic Insert: Arid3a
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169309 Copy
Species: Mus musculus
Genetic Insert: Soma-jGCaMP8m
Vector Backbone Description: Vector Backbone:pGP-AAV-syn-jGCaMP8m-WPRE; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_169257 Copy
Species: Mus musculus
Genetic Insert: Soma-jGCaMP8f
Vector Backbone Description: Vector Backbone:pGP-AAV-syn-jGCaMP8f-WPRE; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_169258 Copy
Species: Mus musculus
Genetic Insert: VP64-Arid3a
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169313 Copy
Species: Mus musculus
Genetic Insert: Arid3a-3'UTR-WT
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169299 Copy
Species: Mus musculus
Genetic Insert: DdCBE for ND5 mutation
Vector Backbone Description: Backbone Size:3726; Vector Backbone:p3s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33608520
Proper citation: RRID:Addgene_169212 Copy
Species: Mus musculus
Genetic Insert: Super ecliptic pHluorin (SEP)
Vector Backbone Description: Vector Backbone:pMiniT; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33689678
Proper citation: RRID:Addgene_169425 Copy
Species: Mus musculus
Genetic Insert: spCas9
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33689678
Proper citation: RRID:Addgene_169420 Copy
Species: Mus musculus
Genetic Insert: Super ecliptic pHluorin (SEP)
Vector Backbone Description: Vector Backbone:pMiniT; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33689678
Proper citation: RRID:Addgene_169421 Copy
Species: Mus musculus
Genetic Insert: Super ecliptic pHluorin (SEP)
Vector Backbone Description: Vector Backbone:pMiniT; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33689678
Proper citation: RRID:Addgene_169418 Copy
Species: Mus musculus
Genetic Insert: Myc
Vector Backbone Description: Vector Backbone:pMiniT; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33689678
Proper citation: RRID:Addgene_169419 Copy
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