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Species: Homo sapiens
Genetic Insert: SETDB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27147599
Comments: Open state: NO stop codon immediately follows insert. Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
Proper citation: RRID:Addgene_82291 Copy
Species: Homo sapiens
Genetic Insert: shRNA to target Fatty Acid Synthase (FASN)
Vector Backbone Description: Backbone Size:7085; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: This plasmid was created from Addgene scramble shRNA Plasmid #1864
Proper citation: RRID:Addgene_82327 Copy
Species: Homo sapiens
Genetic Insert: STK11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27147599
Comments: Open state: NO stop codon immediately follows insert. Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
Proper citation: RRID:Addgene_82320 Copy
Species: Homo sapiens
Genetic Insert: STAT3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27147599
Comments: Open state: NO stop codon immediately follows insert. Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
Proper citation: RRID:Addgene_82235 Copy
Species: Homo sapiens
Genetic Insert: PIK3CB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27147599
Comments: Open state: NO stop codon immediately follows insert. Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
Proper citation: RRID:Addgene_82221 Copy
Species: Mus musculus
Genetic Insert: mouse immunoglobulin heavy chain IgG3 isotype
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4501; Vector Backbone:pFUSEss-CHIg-mG3; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27484487
Proper citation: RRID:Addgene_82356 Copy
Species: Mus musculus
Genetic Insert: mouse immunoglobulin heavy chain IgG1 isotype
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4492; Vector Backbone:pFUSEss-CHIg-mG1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27484487
Proper citation: RRID:Addgene_82357 Copy
Species: Mus musculus
Genetic Insert: mouse immunoglobulin kappa light chain, variable fragment derived from clone M18
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:3862; Vector Backbone:pFUSE2ss-CLIg-mK; Vector Types:Mammalian Expression; Bacterial Resistance:Blasticidin
Defining Citation: PMID:27484487
Proper citation: RRID:Addgene_82358 Copy
Species: methanococcus jannaschii
Genetic Insert: Methanococcus jannaschii aaRS for sulfotyrosine
Vector Backbone Description: Backbone Size:4056; Vector Backbone:pUltra; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26611838
Proper citation: RRID:Addgene_82417 Copy
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pACSA; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27481676
Proper citation: RRID:Addgene_82372 Copy
Genetic Insert: tdTomato (lacks stop codon)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82404 Copy
Species: Homo sapiens
Genetic Insert: human SF3B1-WT
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27524419
Proper citation: RRID:Addgene_82576 Copy
Species: Synthetic
Genetic Insert: mNeonGreen2(1-10)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28851864
Proper citation: RRID:Addgene_82610 Copy
Species: Synthetic
Genetic Insert: mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
Vector Backbone Description: Backbone Size:5296; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28851864
Comments: In the mNG2(1-10) fragment, mutations are K128M, S142T, R150M, G172V and K213M; in the mNG2(11) fragment, mutation is V15M.
Proper citation: RRID:Addgene_82611 Copy
Species: Homo sapiens
Genetic Insert: Ple261
Vector Backbone Description: Vector Backbone:pJ248; Vector Types:Pleiades Promoter Project [sic, Pleaides Plieades]; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31323276
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Please see the following publication for more information:
rAAV-compatible MiniPromoters for restricted expression in the brain and eye.
de Leeuw et al. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. PMID 27164903
Proper citation: RRID:Addgene_82563 Copy
Species: Synthetic
Genetic Insert: sfCherry2(1-10)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28851864
Proper citation: RRID:Addgene_82603 Copy
Genetic Insert: scFvGCN4sfGFPTET1CD
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27571369
Proper citation: RRID:Addgene_82561 Copy
Genetic Insert: FKBP-HA (no pA)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2R-P3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82598 Copy
Species: Synthetic
Genetic Insert: H2B-mCherry and H2B-Citrine. HygroR
Vector Backbone Description: Backbone Size:1950; Vector Backbone:SMX DEST vector Addgene 62399; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27152616
Proper citation: RRID:Addgene_82478 Copy
Species: Aequorea victoria
Genetic Insert: Green Fluorescence Protein with 3 UAG codons
Vector Backbone Description: Backbone Marker:Bio-Rad; Backbone Size:4651; Vector Backbone:pGLO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26611838
Proper citation: RRID:Addgene_82501 Copy
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