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Genetic Insert: E. faecium Com15 recT under an IPTG inducible promoter, with lacI
Vector Backbone Description: Backbone Marker:Luciano Marraffini; Backbone Size:3729; Vector Backbone:plZ12-Spec; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34232061
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.09.01.278044v2 for BioRxiv preprint.
Proper citation: RRID:Addgene_167546 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167603 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167602 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167601 Copy
Vector Backbone Description: Backbone Size:4656; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD134.99, Ligation number L4753.
Proper citation: RRID:Addgene_1676 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167605 Copy
Species: Shigella flexneri
Genetic Insert: rfb region of Shigella flexneri serotype 2a
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHC79; Vector Types:Cosmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1724058
Proper citation: RRID:Addgene_167552 Copy
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pWSK29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9680210
Proper citation: RRID:Addgene_167556 Copy
Species: Vibrio cholerae
Genetic Insert: Vibrio cholerae O antigens genes of the Ogawa serotype
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHC79; Vector Types:Cosmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2426198
Comments: Please note that Addgene NGS found a duplication of the AmpR/ori region as compared to the depositor's original sequence. The depositor has confirmed that they do not expect any issues to arise from the duplication.
Proper citation: RRID:Addgene_167550 Copy
Species: Xenopus laevis
Genetic Insert: XDpr-pt mutant
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: Description: Full length Xenopus Dapper (clone XE151), with last four amino acids mutated to MNTV instead of MMTV in the PDZ binding domain, in pCS2+. Xba1 site is NOT methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16768 Copy
Species: Homo sapiens
Genetic Insert: ELF1
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167745 Copy
Species: Homo sapiens
Genetic Insert: ELF4
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167748 Copy
Species: Xenopus laevis
Genetic Insert: Dishevelled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: HA tagged Xenopus Dishevelled with a triple point mutation outside of the PDZ binding domain, that does not block binding to Dapper.
Notes for Use: Linearize with either Not1 or Asp718
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461
Proper citation: RRID:Addgene_16779 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:34717688
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167694 Copy
Species: Xenopus laevis
Genetic Insert: GFP-XDpr
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: 5' GFP tag on full length Xenopus Dapper (clone XE151) in pCS2+. Xba1 site is DAM methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16770 Copy
Species: Homo sapiens
Genetic Insert: IRF5
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167762 Copy
Species: Homo sapiens
Genetic Insert: STA5B
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167800 Copy
Species: Homo sapiens
Genetic Insert: IRF1
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167760 Copy
Species: Homo sapiens
Genetic Insert: TEAD2
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167804 Copy
Species: Homo sapiens
Genetic Insert: STAT4
Vector Backbone Description: Vector Backbone:MAC-tag-N (#108078); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35140242
Proper citation: RRID:Addgene_167802 Copy
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