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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
R5 MCS
 
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RRID:Addgene_51732 MCS Ampicillin PMID:22718970 Backbone Marker:Promega; Backbone Size:4600; Vector Backbone:pGl4.13; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
p413TEF-SirT1
 
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1+ mentions
RRID:Addgene_51746 SirT1 Homo sapiens Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 2
p413TEF-Sir2
 
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RRID:Addgene_51742 Sir2 Saccharomyces cerevisiae Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
ESAM-bio-His
 
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RRID:Addgene_51751 ESAM Homo sapiens Ampicillin PMID:25713122 Article ref 20 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
EPHB1-bio-His
 
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1+ mentions
RRID:Addgene_51750 EPHB1 Homo sapiens Ampicillin PMID:25713122 Article ref 19 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 1
ICAM5-bio-His
 
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RRID:Addgene_51759 ICAM5 Homo sapiens Ampicillin PMID:25713122 Article ref 28 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
ICAM2-bio-His
 
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RRID:Addgene_51758 ICAM2 Homo sapiens Ampicillin PMID:25713122 Article ref 27 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
FCGR2a-bio-His
 
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RRID:Addgene_51754 FCGR2a Homo sapiens Ampicillin PMID:25713122 Article ref 22 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
lentiCRISPR - EGFP sgRNA 3
 
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1+ mentions
RRID:Addgene_51762 Cas9 Synthetic Ampicillin PMID:24336571 gRNA target sequence GGCCACAAGTTCAGCGTGTC These six EGFP-targeting lentiCRISPRs have EGFP-targeting sgRNAs cloned into the lentiCRISPR backbone plasmid (Addgene plasmid 49535) and are used in Fig 1B of Shalem*, Sanjana* et al (2014). If you want to clone your own targeting sequences, please use the lentiCRISPR backbone plasmid (Addgene plasmid 49535), as the sgRNA Type IIs cloning site is not present in these plasmids. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information. Backbone Marker:Zhang lab; Vector Backbone:lentiCRISPR (pXPR_001), Addgene plasmid #49535; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 2
pCDNA3 T7 Jip1(delta JBD)
 
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RRID:Addgene_51767 Jnk interacting protein 1 Mus musculus Ampicillin PMID:9235893 Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin deleted 126-263 2026-08-29 01:06:43 0
lentiCRISPR - EGFP sgRNA 4
 
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1+ mentions
RRID:Addgene_51763 Cas9 Synthetic Ampicillin PMID:24336571 gRNA target sequence GGAGCGCACCATCTTCTTCA These six EGFP-targeting lentiCRISPRs have EGFP-targeting sgRNAs cloned into the lentiCRISPR backbone plasmid (Addgene plasmid 49535) and are used in Fig 1B of Shalem*, Sanjana* et al (2014). If you want to clone your own targeting sequences, please use the lentiCRISPR backbone plasmid (Addgene plasmid 49535), as the sgRNA Type IIs cloning site is not present in these plasmids. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information. Backbone Marker:Zhang lab; Vector Backbone:lentiCRISPR (pXPR_001), Addgene plasmid #49535; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 4
KIAA-bio-His
 
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RRID:Addgene_51808 KIAA Homo sapiens Ampicillin PMID:25713122 Article ref 31 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
JAM3-bio-His
 
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RRID:Addgene_51807 JAM3 Homo sapiens Ampicillin PMID:25713122 Article ref 30 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
IL6ST-bio-His
 
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RRID:Addgene_51806 IL6ST Homo sapiens Ampicillin PMID:25713122 Article ref 29 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
pfast bac cry1myc
 
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RRID:Addgene_51892 cry1 Mus musculus Ampicillin PMID:23452855 Backbone Marker:Invitrogen; Vector Backbone:pfastbac; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:06:44 0
pfast bac Rbx-HA
 
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RRID:Addgene_51893 Rbx1 Homo sapiens Ampicillin PMID:23452855 Vector Backbone:pfastbac; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:06:44 0
PRNP-bio-His
 
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RRID:Addgene_51811 PRNP Homo sapiens Ampicillin PMID:25713122 Article ref 88 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 0
Alpha MHC-DN.JNK2
 
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RRID:Addgene_51931 DN JNK2 Mus musculus Ampicillin PMID:14517246 Addgene sequencing found the following additional mutations: S2G, S6C and G8S, they not thought to effect the function of the insert. Vector Backbone:alphaMHC vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin dominant negative: the dual phosphorylation motif TPY was changed to APF 2026-08-29 01:06:44 0
Syn-ATP
 
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1+ mentions
RRID:Addgene_51819 Chimeric Synaptophysin-mCherry-Luciferase Rattus norvegicus Ampicillin PMID:24529383 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Within WT luciferase gene, changed Threonine 214 to Alanine, changed Alanine 215 to Leucine, changed Isoleucine 232 to Alanine, changed Phenylalanine 295 to Leucine, changed Glutamic Acid 354 to Lysine, changed Isoleucine 423 to Leucine, and changed Aspartic Acid 436 to Glycine. 2026-08-29 01:06:43 4
pCS2+hSpCas9
 
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10+ mentions
RRID:Addgene_51815 humanized S.pyogenes Cas9 Ampicillin PMID:24728957 This plasmid is modified from pX330-U6-Chimeric BB-CBh-hSpCas9 originated from Feng Zhang Lab. Backbone Size:4071; Vector Backbone:pCS2; Vector Types:Mammalian Expression, CRISPR, medaka, zebrafish; Bacterial Resistance:Ampicillin 2026-08-29 01:06:43 29

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