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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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BAZ2AA-c002 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53623 | BAZ2AA | Other | Kanamycin | T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4LZ2/ Use BL21(DE3)-R3-pRARE2 strain for expression. | Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Bacterial expression for structure determination; may not be full ORF, see comments | 2026-08-29 01:06:59 | 3 | |
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Nano-lantern(cAMP-1.6)/pcDNA3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53594 | Nano-lantern-based luminescent cAMP indicator | Synthetic | Ampicillin | PMID:23232392 | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:06:58 | 3 | ||
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CYYR1_pCSdest Resource Report Resource Website 1+ mentions |
RRID:Addgene_53794 | CYYR1 | Homo sapiens | Ampicillin | PMID:29760202 | BC036761 | Backbone Marker:Lawson Lab (Addgene Plasmid 22423); Backbone Size:4095; Vector Backbone:pCSDest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:07:00 | 1 | |
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SAMSN1_pCSdest Resource Report Resource Website 1+ mentions |
RRID:Addgene_53792 | SAMSN1 | Homo sapiens | Ampicillin | PMID:29760202 | NM_022136 | Backbone Marker:Lawson Lab (Addgene Plasmid 22423); Backbone Size:4095; Vector Backbone:pCSDest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:07:01 | 1 | |
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pBRα Resource Report Resource Website 1+ mentions |
RRID:Addgene_53731 | αNTD | E. coli | Ampicillin | PMID:9121589 | This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642 | Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:07:00 | 1 | |
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pMiR-HPV16-E7 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53696 | HPV16-E7 | Human papillomavirus | Kanamycin | PMID:24708873 | Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin | 2026-08-29 01:07:00 | 1 | ||
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pMiR-HPV16-E1(partial) Resource Report Resource Website 1+ mentions |
RRID:Addgene_53699 | HPV16-E1(partial) | Human papillomavirus | Kanamycin | PMID:24708873 | Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin | 2026-08-29 01:07:00 | 1 | ||
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pACλCI-β-flap (831-1057) Resource Report Resource Website 1+ mentions |
RRID:Addgene_53733 | λCI-β-flap fusion | Other | Chloramphenicol | PMID:9121589 | Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details. This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642 | Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:07:00 | 1 | |
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pcDNA3/Flag-WTAP Resource Report Resource Website 1+ mentions |
RRID:Addgene_53741 | Wilms tumor 1 associated protein | Homo sapiens | Ampicillin | PMID:24316715 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:07:00 | 8 | ||
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pCS-membrane-ceruleanFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_53749 | 2x membrane linker | Other | Ampicillin | PMID:19378112 | Use NotI/SP6 for RNA to inject. Put XbaI/AgeI membrane linker in place of histone2B in XbaI/AgeI cut pCS-H2Bcerulean. This puts membrane-cerulean between XbaI and SnaBI of pCS. | Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:07:00 | 2 | |
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pCS-H2B-EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_53744 | histone H2B | Homo sapiens | Ampicillin | PMID:19378112 | Cut with NotI and use SP6 for sense RNA. | Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:07:00 | 6 | |
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pEGFP-C3-Sec8 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53758 | Sec8 | Homo sapiens | Kanamycin | PMID:24389102 | Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:07:00 | 1 | ||
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pEGFP-C3-Sec10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53759 | Sec10 | Homo sapiens | Kanamycin | PMID:24389102 | Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:07:00 | 1 | ||
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pEGFP-C3-Sec5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53756 | Sec5 | Homo sapiens | Kanamycin | PMID:24389102 | Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:07:00 | 2 | ||
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pEGFP-C3-Sec3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53755 | Sec3 | Homo sapiens | Kanamycin | PMID:24389102 | Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:07:00 | 6 | ||
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pEGFP-C3-Exo84 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53762 | Exo84 | Homo sapiens | Kanamycin | PMID:24389102 | Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:07:00 | 3 | ||
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pcDNA4-ATR-C Resource Report Resource Website 1+ mentions |
RRID:Addgene_53769 | ATR | Homo sapiens | Ampicillin | PMID:12011431 | **R1631H polymorphism--not known to affect function | Backbone Marker:Invitrogen; Vector Backbone:pcDNA4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | C-terminal domain, aa 1398-2644* | 2026-08-29 01:07:00 | 2 |
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pcDNA4-ATR-M Resource Report Resource Website 1+ mentions |
RRID:Addgene_53768 | ATR | Homo sapiens | Ampicillin | PMID:12011431 | *R1631H polymorphism--not known to affect function | Backbone Marker:Invitrogen; Vector Backbone:pcDNA4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | middle domain, aa 593-1700* | 2026-08-29 01:07:00 | 2 |
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SLC5A3_pCSdest Resource Report Resource Website 1+ mentions |
RRID:Addgene_53836 | SLC5A3 | Homo sapiens | Ampicillin | PMID:29760202 | BC146536 | Backbone Marker:Lawson Lab (Addgene Plasmid 22423); Backbone Size:4095; Vector Backbone:pCSDest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:07:01 | 1 | |
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LTN1_pCSdest Resource Report Resource Website 1+ mentions |
RRID:Addgene_53855 | LTN1 | Homo sapiens | Ampicillin | PMID:29760202 | BC150284 | Backbone Marker:Lawson Lab (Addgene Plasmid 22423); Backbone Size:4095; Vector Backbone:pCSDest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:07:01 | 1 |
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