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Genetic Insert: MCS with F2A and T2A
Vector Backbone Description: Backbone Marker:Agilent; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62621 Copy
Species: Homo sapiens
Genetic Insert: CHRFAM7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25056953
Proper citation: RRID:Addgene_62638 Copy
Species: E. coli
Genetic Insert: Thr251Gly-EcPheRS
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4541; Vector Backbone:pQE-80L-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25691744
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_62598 Copy
Genetic Insert: exo, beta, gam, sgRNA
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26463009
Proper citation: RRID:Addgene_62654 Copy
Genetic Insert: Cas9 nuclease
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26463009
Comments: Please note that this plasmid runs as a dimer (>13kb). While this may reduce DNA yield, the plasmid still functions as expected.
Proper citation: RRID:Addgene_62655 Copy
Genetic Insert: exo, beta, gam, sgRNA
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26463009
Proper citation: RRID:Addgene_62656 Copy
Species: Synthetic
Genetic Insert: mtZFN(-)2G
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24567072
Proper citation: RRID:Addgene_62799 Copy
Species: Homo sapiens
Genetic Insert: CenpA
Vector Backbone Description: Vector Backbone:N1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62742 Copy
Species: Mus musculus
Genetic Insert: Talin
Vector Backbone Description: Vector Backbone:C1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62763 Copy
Species: Homo sapiens
Genetic Insert: PML-IV
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205865
Comments: The PML isoform in this plasmid is referred to as PML-IV according to the nomenclature established by Jensen, Shiels and Freemont Oncogene. 2001 Oct 29;20(49):7223-33. The GenBank nomenclature for PML-IV is PML isoform 6 (GenBank ID NM_002675.3).
Proper citation: RRID:Addgene_62804 Copy
Species: Synthetic
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pFG001; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_62816 Copy
Species: Synthetic
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pFG018; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_62817 Copy
Species: Synthetic
Genetic Insert: SYNZIP3
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4892; Vector Backbone:pQE-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22558529
Proper citation: RRID:Addgene_80659 Copy
Species: Synthetic
Genetic Insert: SYNZIP2
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4892; Vector Backbone:pQE-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22558529
Proper citation: RRID:Addgene_80658 Copy
Species: Other
Genetic Insert: pME-mKate2 no stop
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2518; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_80806 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang lab (Plasmid #71814); Backbone Size:8506; Vector Backbone:eSpCas9(1.1); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28179459
Proper citation: RRID:Addgene_80768 Copy
Species: Synthetic
Genetic Insert: PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
Vector Backbone Description: Backbone Marker:Kazuhisa Nakayama Lab. (Addgene plasmid #80766); Backbone Size:4761; Vector Backbone:pDonor-tBFP-NLS-Neo; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28179459
Proper citation: RRID:Addgene_80767 Copy
Vector Backbone Description: Backbone Size:5018; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19716372
Comments: Annotated sequence available from:
www.ebi.ac.uk/ena
accession number LT615370
Proper citation: RRID:Addgene_80797 Copy
Species: Synthetic
Genetic Insert: Monomeric streptavidin
Vector Backbone Description: Backbone Marker:Homemade; Backbone Size:5300; Vector Backbone:pET-IG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26979420
Proper citation: RRID:Addgene_80706 Copy
Species: Synthetic
Genetic Insert: SYNZIP4
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4892; Vector Backbone:pQE-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22558529
Proper citation: RRID:Addgene_80660 Copy
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