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Species: Drosophila melanogaster
Genetic Insert: UAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
Vector Backbone Description: Backbone Marker:Pfeiffer et al; Backbone Size:7831; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21297621
Comments: This plasmid contains mTFP1.0, and not EBFP2, as suggested by the linear plasmid map image file.
Please note that Addgene's sequencing results identified a few discrepancies when compared to GenBank ID JF267350. The depositing laboratory states that these differences are not a concern for the function of the plasmid. The full plasmid sequence has been assembled and not entirely confirmed by sequencing. Additional discrepancies may exist.
This plasmid was constructed using the following restriction enyzmes:
Bgl II / Not I - loxP sites
Not I / Xho I - EGFP-HSV
Xho I / Kpn I - mTFP1.0-HA
Kpn I / Xba I - mKO2-V5
NotI is not a unique cutter--it is present twice in the plasmid. The XbaI site has been confirmed by Addgene's sequencing results, but it not present in the full plasmid sequence.
Proper citation: RRID:Addgene_29327 Copy
Species: Drosophila melanogaster
Genetic Insert: phiC31 attB, hsp70::w
Vector Backbone Description: Backbone Size:2881; Vector Backbone:pUC; Vector Types:Drosophila transgenesis; Bacterial Resistance:Ampicillin
Comments: w[+] is hsp70 basal promoter; gives full w+ (red eyes), without heat shock.
Proper citation: RRID:Addgene_30326 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila melanogaster PRL
Vector Backbone Description: Backbone Marker:Novagen Inc., Madison, WI; Backbone Size:5708; Vector Backbone:pET-15B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36822330
Proper citation: RRID:Addgene_166418 Copy
Species: Drosophila melanogaster
Genetic Insert: Dorado
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:6300; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190640 Copy
Species: Drosophila melanogaster
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:6300; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190641 Copy
Species: Drosophila melanogaster
Genetic Insert: Dorado
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:6300; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190638 Copy
Species: Drosophila melanogaster
Genetic Insert: Dorado
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:6300; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190608 Copy
Species: Drosophila melanogaster
Genetic Insert: dora (CG34401) sgRNA 1
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:10615; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190700 Copy
Species: Drosophila melanogaster
Genetic Insert: dora (CG34401) sgRNA 2
Vector Backbone Description: Backbone Marker:Ji-Long Liu; Backbone Size:10615; Vector Backbone:pAc-sgRNA-Cas9; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36150386
Proper citation: RRID:Addgene_190701 Copy
Species: Drosophila melanogaster
Genetic Insert: DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27773676
Comments: DmThor sequence given by NCBI: NM_057947.4.; DmeIF4Ga ORF extracted from NCBI: NM_079901.3. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148512 Copy
Species: Drosophila melanogaster
Genetic Insert: DmCaf40_25-291-Y139DG146W
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29255063
Comments: ORF extracted from NCBI: NM_167675.1. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148736 Copy
Species: Drosophila melanogaster
Genetic Insert: DmGe1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815998
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146688 Copy
Species: Drosophila melanogaster
Genetic Insert: DmPABPC1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146368 Copy
Species: Drosophila melanogaster
Genetic Insert: DmLSm8
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: ORF extracted from NCBI: NM_139403.1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146367 Copy
Species: Drosophila melanogaster
Genetic Insert: DmImp8
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: ORF extracted from NCBI: NM_080041.2. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146365 Copy
Species: Drosophila melanogaster
Genetic Insert: DmeIF4A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: ORF extracted from NCBI: NM_164668.1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146361 Copy
Species: Drosophila melanogaster
Genetic Insert: CG5263-3UTR
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: 3' UTR extracted from NCBI: NM_001144453.1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146375 Copy
Species: Drosophila melanogaster
Genetic Insert: DmXRN1_574-1613
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146372 Copy
Species: Drosophila melanogaster
Genetic Insert: DmPop2a-D53AE55A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146370 Copy
Species: Drosophila melanogaster
Genetic Insert: DmAGO1-RC
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146262 Copy
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