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Species: E. coli
Genetic Insert: atpA
Vector Backbone Description: Backbone Marker:TakaraBio; Backbone Size:9138; Vector Backbone:pLVX-TetOne-bsd; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40894546
Comments: Please visit https://doi.org/10.1101/2025.08.12.670003 for bioRxiv preprint.
Proper citation: RRID:Addgene_248065 Copy
Species: E. coli
Genetic Insert: C5 protein of E. coli RNase P
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40858540
Comments: When using this plasmid, please also cite the original paper:
Fukunaga J, Gouda M, Umeda K, Ohno S, Yokogawa T, Nishikawa K. Use of RNase P for efficient preparation of yeast tRNATyr transcript and its mutants. J Biochem. 2006 Jan;139(1):123-7. doi: 10.1093/jb/mvj005. PMID: 16428327
Proper citation: RRID:Addgene_246823 Copy
Species: E. coli
Genetic Insert: E. coli tRNA arrays
Vector Backbone Description: Backbone Marker:Twist bioscience; Backbone Size:2959; Vector Backbone:pTwist vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40858540
Proper citation: RRID:Addgene_246822 Copy
Species: E. coli
Genetic Insert: IS621 Donor
Vector Backbone Description: Vector Backbone:Rep101 Ts - pSC101 Ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38926615
Comments: Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint.
Proper citation: RRID:Addgene_222731 Copy
Species: E. coli
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41535170
Proper citation: RRID:Addgene_254018 Copy
Species: E. coli
Genetic Insert: L3S3P11
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 4 pasts are terminators in the CTK system
Please note: This plasmid contains a C nucleotide insertion in L3S3P11. This insertion is not known to impact plasmid function.
Proper citation: RRID:Addgene_248472 Copy
Species: E. coli
Genetic Insert: E. coli tyrosyl tRNA synthetase VSMA mutant
Vector Backbone Description: Backbone Size:1974; Vector Backbone:pBK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41039161
Proper citation: RRID:Addgene_247683 Copy
Species: E. coli
Genetic Insert: Chloramphenicol acetyl transferase fusion protein
Vector Backbone Description: Vector Backbone:CCLMPC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40536430
Proper citation: RRID:Addgene_237500 Copy
Species: E. coli
Genetic Insert: T7-proOmpA-pep99
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2025.12.09.688994 for bioRxiv preprint.
Proper citation: RRID:Addgene_250510 Copy
Species: E. coli
Genetic Insert: T7-secY(I408G)EG
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2025.12.09.688994 for bioRxiv preprint.
Proper citation: RRID:Addgene_250516 Copy
Species: E. coli
Genetic Insert: T7-secYEG
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2025.12.09.688994 for bioRxiv preprint.
Proper citation: RRID:Addgene_250513 Copy
Species: E. coli
Genetic Insert: T7-secY(P84L)EG
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2025.12.09.688994 for bioRxiv preprint.
Proper citation: RRID:Addgene_250512 Copy
Species: E. coli
Genetic Insert: RBS-S2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2d parts are Ribosome Binding Sites (RBSs) in the CTK system
Proper citation: RRID:Addgene_248439 Copy
Species: E. coli
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 3 parts coding sequences in the CTK system
Proper citation: RRID:Addgene_248442 Copy
Species: E. coli
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 3 parts coding sequences in the CTK system
Proper citation: RRID:Addgene_248443 Copy
Species: E. coli
Genetic Insert: ameR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 3 parts coding sequences in the CTK system
Proper citation: RRID:Addgene_248445 Copy
Species: E. coli
Genetic Insert: RBS-S3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2d parts are Ribosome Binding Sites (RBSs) in the CTK system
Proper citation: RRID:Addgene_248440 Copy
Species: E. coli
Genetic Insert: PBAD-RiboJ-B0064
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2 parts are transcriptional and translational control parts in the CTK system
Proper citation: RRID:Addgene_248494 Copy
Species: E. coli
Genetic Insert: PTet-RiboJ-B0064
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2 parts are transcriptional and translational control parts in the CTK system
Proper citation: RRID:Addgene_248495 Copy
Species: E. coli
Genetic Insert: L3S2P21
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 4b pasts are terminators in the CTK system
Proper citation: RRID:Addgene_248475 Copy
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