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Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65594 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65593 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65596 Copy
Species: Caenorhabditis elegans
Genetic Insert: Let-60
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Proper citation: RRID:Addgene_66861 Copy
Species: Caenorhabditis elegans
Genetic Insert: mKate2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD287 (https://www.addgene.org/70685/). The full sequence for this plasmid can still be found by clicking the link next to "Depositor Sequences".
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
Proper citation: RRID:Addgene_66826 Copy
Species: Caenorhabditis elegans
Genetic Insert: TagRFP-T-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD286 (https://www.addgene.org/70684/). The full sequence for this plasmid can still be found by clicking the link next to "Addgene Sequences" and scrolling to the bottom of the page for "Full Addgene NGS sequence".
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
Proper citation: RRID:Addgene_66825 Copy
Species: Caenorhabditis elegans
Genetic Insert: CST-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Comments: Addgene sequencing results find amino acid changes S48N and H50R compared to available CST-1 reference sequences.
Proper citation: RRID:Addgene_66859 Copy
Species: Caenorhabditis elegans
Genetic Insert: YAP-1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396260
Comments: I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine.
Proper citation: RRID:Addgene_66858 Copy
Species: Caenorhabditis elegans
Genetic Insert: YAP-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396260
Comments: Addgene sequencing results find amino acid changes Q48R and Y213F compared to available YAP-1 reference sequences.
Proper citation: RRID:Addgene_66854 Copy
Species: Caenorhabditis elegans
Genetic Insert: RSF-1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Comments: I performed PCR on C.elegans genome to isolate the genome of RSF-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon.
Proper citation: RRID:Addgene_66863 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3::NLS::GCaMP6s
Vector Backbone Description: Vector Backbone:Not Available; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26712014
Proper citation: RRID:Addgene_68119 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:7606; Vector Backbone:pCFJ356; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69149 Copy
Species: Caenorhabditis elegans
Genetic Insert: TagBFP2
Vector Backbone Description: Backbone Marker:Andrew Fire; Backbone Size:3417; Vector Backbone:pPD49_26; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69259 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69257 Copy
Species: Caenorhabditis elegans
Genetic Insert: ceBFP SEC 3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: The plasmid was made based on pDD268 from Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.178335.
Proper citation: RRID:Addgene_69340 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-16
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid 1497); Backbone Size:4406; Vector Backbone:pPD95.81; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23805378
Comments: The D->V point mutation in DAF-16 has no functional consequence.
Proper citation: RRID:Addgene_52032 Copy
Species: Caenorhabditis elegans
Genetic Insert: fli-1
Vector Backbone Description: Backbone Marker:Fire lab; Backbone Size:4487; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18485202
Proper citation: RRID:Addgene_52348 Copy
Species: Caenorhabditis elegans
Genetic Insert: swan-1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6299; Vector Backbone:pCMV-3xFLAG; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16980389
Proper citation: RRID:Addgene_52349 Copy
Species: Caenorhabditis elegans
Genetic Insert: rack-1
Vector Backbone Description: Backbone Marker:Fire lab; Backbone Size:4487; Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21124943
Proper citation: RRID:Addgene_52351 Copy
Species: Caenorhabditis elegans
Genetic Insert: Dre-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5404; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396
Proper citation: RRID:Addgene_52517 Copy
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