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Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22245580
Comments: NOTE: there is a second, non-annotated EcoRI site present in this plasmid. The function of the plasmid will not be impaired; however,
you cannot use the EcoRI site in the MCS for cloning.
Proper citation: RRID:Addgene_43796 Copy
Species: Homo sapiens
Genetic Insert: LIN28B
Vector Backbone Description: Backbone Size:5372; Vector Backbone:pFRT/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23481595
Comments: Note that the Flag and HA tags are not in frame in the depositor's assembled sequence, but Addgene's quality control sequence shows they are in frame.
Proper citation: RRID:Addgene_43798 Copy
Vector Backbone Description: Backbone Marker:John Mekalanos lab, Harvard Medical School (PMID: 2836362); Backbone Size:5720; Vector Backbone:pGP704; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9511756
Comments: The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination.
Plasmid pRE107 was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis.
The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers.
Note: Addgene's NGS results found that a large portion of the plasmid backbone (containing the oriT feature) is in the opposite orientation compared to the depositor's reference sequence.
Proper citation: RRID:Addgene_43829 Copy
Species: Homo sapiens
Genetic Insert: human WNT11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43824 Copy
Species: Homo sapiens
Genetic Insert: WNT8A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43818 Copy
Species: Homo sapiens
Genetic Insert: WNT8B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43819 Copy
Species: Homo sapiens
Genetic Insert: WNT4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43812 Copy
Species: Homo sapiens
Genetic Insert: WNT5A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43813 Copy
Species: Homo sapiens
Genetic Insert: human WNT7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43816 Copy
Species: Homo sapiens
Genetic Insert: WNT2B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43808 Copy
Species: Homo sapiens
Genetic Insert: Human Optimized S. pyogenes Cas9
Vector Backbone Description: Backbone Size:6670; Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23460208
Proper citation: RRID:Addgene_43804 Copy
Species: Mus musculus
Genetic Insert: Hes1 Promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7906273
Proper citation: RRID:Addgene_43806 Copy
Genetic Insert: SID4X Domain
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22828628
Comments: This enhanced SID domain (SID4X domain) is to be used with TALE effectors to create TALE repressors, as described in Cong et al. Nature Communications. This new domain replaces the previous SID domain reported in the original article.
Proper citation: RRID:Addgene_43882 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6841; Vector Backbone:pET, pETM44; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43986 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 3
Vector Backbone Description: Backbone Marker:Shao-Cong Sun; Vector Backbone:pcDNA-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15084608
Comments: pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.
Please note that the hTRAF3 insert in this plasmid contains V274D and S393G mutations when compared to GenBank entry NP_663777.1
Proper citation: RRID:Addgene_44033 Copy
Genetic Insert: AGA1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9181578
Proper citation: RRID:Addgene_44036 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-Tax-3'UTR
Vector Backbone Description: Backbone Marker:Dr. Inder Verma; Vector Backbone:pCLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11553609
Comments: Transfer of BamHI-BamHI fragment covering 5'UTR-Tax-3'UTR from pCMV4-Tax (from Dr. Warner Greene) to the BamHI site of pCLXSN (from Dr. Verma Inder).
Proper citation: RRID:Addgene_44038 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6406; Vector Backbone:pET, pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Note that the 6x His GST double tag tends to increase the formation of insoluble protein aggregates.
Proper citation: RRID:Addgene_43983 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5749; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Note that depending on the protein, the 10x His tag can reduce or abolish expression.
Proper citation: RRID:Addgene_43975 Copy
Species: Mus musculus
Genetic Insert: mHIF-2α (P405A/P530V/N851A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17804822
Comments: This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-2α.
Full-length murine HIF-2α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites.
A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-2α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 405, Proline 530 and Asparagine 851. The newly constructed plasmid was sequenced to confirm encoding of the correct protein.
This plasmid has an A94V polymorphism that does not affect protein function.
Alternate plasmid names: HIF-2α triple mutant (TM); pcDNA3 HIF-2α TM
Proper citation: RRID:Addgene_44027 Copy
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