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Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4514; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: kanMX4 in pRS400 was replaced with natMX4 from pAG25.
Proper citation: RRID:Addgene_35480 Copy
Species: Synthetic
Genetic Insert: Vx3K0
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6300; Vector Backbone:pQCXIX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22306808
Comments: Please note that the C89S mutation makes the mammalian version different than the bacterially expressed version, but this does not change plasmid function.
Proper citation: RRID:Addgene_35527 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4328; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: kanMX4 in pRS400 was replaced with bleMX4 from pUG66.
Proper citation: RRID:Addgene_35478 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments:
Proper citation: RRID:Addgene_35510 Copy
Species: Halorubrum sp. TP009
Genetic Insert: eArchT 3.0-EYFP
Vector Backbone Description: Backbone Size:9245; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments: The enhanced trafficking signals allow for better membrane targeting in mammalian cells resulting in 3-5 fold increase in currents.
Please note that there may be several sequence discrepancies between depositor's reference sequence and Addgene's quality control sequence. These changes are in vector regions only and should not affect plasmid function.
Proper citation: RRID:Addgene_35513 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4960; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: kanMX4 in pRS400 was replaced with hphMX4 from pAG32.
Proper citation: RRID:Addgene_35479 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5885; Vector Backbone:pRS42x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: ADE1 has been modified to remove BamHI, EcoRI, SalI and XbaI sites without altering the amino acid sequence of the Ade1 protein.
Proper citation: RRID:Addgene_35472 Copy
Species: H. sodomense
Genetic Insert: Arch3.0-EYFP
Vector Backbone Description: Backbone Size:5379; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments: The enhanced trafficking signals allow for better membrane targeting in mammalian cells resulting in 3-5 fold increase in currents.
Proper citation: RRID:Addgene_35516 Copy
Species: Synthetic
Genetic Insert: ChR1-VChR1 Chimera
Vector Backbone Description: Backbone Size:5385; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments:
Proper citation: RRID:Addgene_35500 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5624; Vector Backbone:pRS42x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: TRP1 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Trp1 protein.
Proper citation: RRID:Addgene_35466 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5618; Vector Backbone:pRS32x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: TRP1 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Trp1 protein.
Proper citation: RRID:Addgene_35465 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5801; Vector Backbone:pRS42x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: HIS3 has been modified to remove HindIII, KpnI and PstI sites without altering the amino acid sequence of the His3 protein.
Proper citation: RRID:Addgene_35464 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2(C128S/D156A)-mCherry
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments: ChR2 variant harboring two amino acid substitutions which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. Like previously published step function opsins, this stabilized step function opsin (SSFO) may be deactivated using yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.
Proper citation: RRID:Addgene_35504 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2(C128S/D156A)-EYFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments: ChR2 variant harboring two amino acid substitutions which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. Like previously published step function opsins, this stabilized step function opsin (SSFO) may be deactivated using yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.
Proper citation: RRID:Addgene_35503 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5711; Vector Backbone:pRS32x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.
Proper citation: RRID:Addgene_35469 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Size:5385; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments: ChR2 variant harboring two amino acid substitutions which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. Like previously published step function opsins, this stabilized step function opsin (SSFO) may be deactivated using yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.
Proper citation: RRID:Addgene_35501 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5797; Vector Backbone:pRS32x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: HIS3 has been modified to remove HindIII, KpnI, and PstI sites without altering the amino acid sequence of the His3 protein.
Proper citation: RRID:Addgene_35463 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6865; Vector Backbone:pRS42x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: ADE2 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Ade2 protein.
Proper citation: RRID:Addgene_35462 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments:
Proper citation: RRID:Addgene_35508 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Proper citation: RRID:Addgene_35507 Copy
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