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Species: Homo sapiens
Genetic Insert: TARBP1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2HA8 http://www.thesgc.org/structures/2HA8/
Proper citation: RRID:Addgene_25505 Copy
Species: Homo sapiens
Genetic Insert: ubc44.001.167
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 1YH2 http://www.thesgc.org/structures/1YH2/
Proper citation: RRID:Addgene_25468 Copy
Species: Homo sapiens
Genetic Insert: GNPNAT1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2HUZ, 2O28, https://www.rcsb.org/structure/2O28
SGC links:
https://www.thesgc.org/structures/2HUZ
https://www.thesgc.org/structures/2O28
Proper citation: RRID:Addgene_25501 Copy
Species: Homo sapiens
Genetic Insert: ubh26.019.099
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 1YQB http://www.thesgc.org/structures/1YQB/
Proper citation: RRID:Addgene_25467 Copy
Species: Homo sapiens
Genetic Insert: Myo D Promoter
Vector Backbone Description: Backbone Marker:Fire et al., 1990; Backbone Size:6110; Vector Backbone:pPD46.21; Vector Types:Mammalian Expression, Reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7720572
Comments: A SalI-XhoI fragment containing the 2.5 kb promoter fragment was cloned into the SalI site of pPD46.21, destroying the XhoI site. The F3 fragment is cloned in reverse orientation.
258 refers to the core enhancer, which is present in this construct, upstream of the 2.5 kb MyoD promoter
Proper citation: RRID:Addgene_25430 Copy
Species: Homo sapiens
Genetic Insert: MAK3
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2OB0 http://www.thesgc.org/structures/2OB0/
Proper citation: RRID:Addgene_25499 Copy
Species: Homo sapiens
Genetic Insert: GAMT
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 1ZX0 http://www.thesgc.org/structures/1ZX0/
Proper citation: RRID:Addgene_25494 Copy
Species: Homo sapiens
Genetic Insert: SRM
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB:
http://www.thesgc.org/structures/2O05/
http://www.thesgc.org/structures/2O06/
http://www.thesgc.org/structures/1ZDZ/
Proper citation: RRID:Addgene_25490 Copy
Species: Homo sapiens
Genetic Insert: SAT
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2FXF http://www.thesgc.org/structures/2FXF/
Proper citation: RRID:Addgene_25493 Copy
Species: Homo sapiens
Genetic Insert: SULT1C1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2ETG
http://www.thesgc.org/structures/2ETG/
Proper citation: RRID:Addgene_25492 Copy
Species: Homo sapiens
Genetic Insert: Myo D 5' flanking sequence
Vector Backbone Description: Backbone Marker:Fire et al., 1990; Backbone Size:6110; Vector Backbone:pPD46.21; Vector Types:Mammalian Expression, Reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11458388
Comments: -24lacZ contains approximately 24 kb of 5' flanking sequences of the human MyoD gene, cloned upstream of the lacZ reporter gene in the vector pPD46.21 (Fire et al., 1990).
MyoD flanking sequences were derived from the CAT reporter construct, -24CAT (Goldhamer et al., 1992), as follows. The unique XhoI site, which is just 3' of human sequences in -24CAT, was Klenow-filled and AscI linkers (New England Biolabs) were added by blunt-end ligation using standard methods. AscI linkers were also added to the unique SmaI site in the polylinker of pPD46.21. The 24-kb fragment was excised from -24CAT by digestion with SalI and AscI, purified by agarose gel electrophoresis and GENECLEAN Xbio-gene), and directionally cloned into pPD46.21 that had been similarly prepared.
The resulting clone contains contiguous MyoD 5' flanking sequences from approximately 24 kb upstream to 170 bp downstream of the MyoD transcriptional start site (-37 relative to the start of translation).
Proper citation: RRID:Addgene_25422 Copy
Species: Homo sapiens
Genetic Insert: Myo D 5' flanking sequence
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pEGFP-1; Vector Types:Mammalian Expression, Reporter construct; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17693064
Comments: The MyoDGFP (-24GFP) transgene was constructed by excising 24 kb of human MyoD 5' flanking sequence from -24lacZ (Chen et al., 2001; Addgene plasmid #25422) by digestion with SalI and AscI (AscI linkers were added to the unique XhoI site at the 3' end of human genomic sequences) and inserting into SalI and AscI (produced by linker addition to the unique SmaI site) sites in the polylinker of pEGFP-1 (Clontech). Prior to subcloning, the NotI site just 3' of EGFP was destroyed by blunt-ending with Klenow, and a new NotI site was introduced by linker addition to the AflII site, which lies 3' of SV40 poly sequences.
Proper citation: RRID:Addgene_25423 Copy
Species: Homo sapiens
Genetic Insert: PANK1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2I7N http://www.thesgc.org/structures/2I7N/
Proper citation: RRID:Addgene_25517 Copy
Species: Homo sapiens
Genetic Insert: CHKA
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2I7Q http://www.thesgc.org/structures/2I7Q/
PDB: 3G15 http://www.thesgc.org/structures/3G15/
PDB: 3F2R http://www.thesgc.org/structures/3F2R/
Proper citation: RRID:Addgene_25515 Copy
Species: Homo sapiens
Genetic Insert: CDC34
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2OB4 http://www.thesgc.org/structures/2OB4/
Proper citation: RRID:Addgene_25598 Copy
Species: Homo sapiens
Genetic Insert: UBCi
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 1ZUO http://www.thesgc.org/structures/1ZUO/
Proper citation: RRID:Addgene_25599 Copy
Species: Homo sapiens
Genetic Insert: HK2
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2NZT http://www.thesgc.org/structures/2NZT/
Proper citation: RRID:Addgene_25529 Copy
Species: Homo sapiens
Genetic Insert: ARL5
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 1Z6Y http://www.thesgc.org/structures/1Z6Y/
Proper citation: RRID:Addgene_25525 Copy
Species: Homo sapiens
Genetic Insert: ARL6
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2H57 http://www.thesgc.org/structures/2H57/
Proper citation: RRID:Addgene_25524 Copy
Species: Homo sapiens
Genetic Insert: ARL5:H14-K177
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2H16 http://www.thesgc.org/structures/2H16/
Proper citation: RRID:Addgene_25526 Copy
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