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Species: Mus musculus
Genetic Insert: Estrogen related receptor, beta
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.); Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412560
Comments: The cDNA sequence of murine Esrrb was amplified from trophoblast stem cells and subcloned into the pCR2.1 vector. From there it was excised via flanking EcoRI sites and inserted into the pLV-tetO vector via the EcoRI. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
Proper citation: RRID:Addgene_71152 Copy
Species: Mus musculus
Genetic Insert: FerH-ffLuc2-eGFP
Vector Backbone Description: Backbone Size:5828; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19175523
Proper citation: RRID:Addgene_71393 Copy
Species: Mus musculus
Genetic Insert: Septin 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21788367
Proper citation: RRID:Addgene_71547 Copy
Species: Mus musculus
Genetic Insert: Cntn2
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71940 Copy
Species: Mus musculus
Genetic Insert: Cntn6
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71944 Copy
Species: Mus musculus
Genetic Insert: Cntn4.2
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71942 Copy
Species: Mus musculus
Genetic Insert: Flrt1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71947 Copy
Species: Mus musculus
Genetic Insert: Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23220192
Comments: For protein production, use BL21 Rosetta (DE3) cells.
Proper citation: RRID:Addgene_71827 Copy
Species: Mus musculus
Genetic Insert: Flrt2
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71948 Copy
Species: Mus musculus
Genetic Insert: Cntn1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71939 Copy
Species: Mus musculus
Genetic Insert: Islr
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71950 Copy
Species: Mus musculus
Genetic Insert: Islr2.b
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71951 Copy
Species: Mus musculus
Genetic Insert: Jam1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71952 Copy
Species: Mus musculus
Genetic Insert: Jam2
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71953 Copy
Species: Mus musculus
Genetic Insert: Lrrc4
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71958 Copy
Species: Mus musculus
Genetic Insert: Lrrc4c
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71959 Copy
Species: Mus musculus
Genetic Insert: Jam4.3
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_71956 Copy
Species: Mus musculus
Genetic Insert: Sema3a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72012 Copy
Species: Mus musculus
Genetic Insert: Sema3a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72010 Copy
Species: Mus musculus
Genetic Insert: Ncam1_1.18.1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: There are 3 mini exons in Ncam. The first mini exon is called "exon Pi" or "VASE". It contains nucleotides that encode the amino acid sequence "ASWTRPEKQE". The second mini exon occurs between exons 13-14. It encodes the amino acid sequence "IALLHK". The third mini exon occurs between exons 14-15 and encodes one amino acid, Lys. Ncam1_1.18.1 contains all three mini exons.
Proper citation: RRID:Addgene_71962 Copy
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