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Species: Homo sapiens
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:860; Vector Backbone:AmpR gene, with start and stop codons; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724390.1 . This plasmid contains an 18 or 19bp truncation in the CMV promoter and was synthesized for making pseudotyped VSV particles. It has been shown that truncations either help expression and /or capture by VSVdeltaG. See https://www.ncbi.nlm.nih.gov/pubmed/19057867 for original paper on VSV pseudotyping, and https://www.cell.com/cell/pdf/S0092-8674(20)30229-4.pdf on more recent efforts with SARS-CoV-2.
Please note: Addgene NGS finds that the AmpR resistance marker is in the opposite orientation in the plasmid relative to the depositor's sequence. As the plasmid was able to grow in media with Ampicillin, this is not expected to affect plasmid function.
Proper citation: RRID:Addgene_149539 Copy
Species: Homo sapiens
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:860; Vector Backbone:AmpR gene, with start and stop codons; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724390.1 . This plasmid contains an 18 or 19bp truncation in the CMV promoter and was synthesized for making pseudotyped VSV particles. It has been shown that truncations either help expression and /or capture by VSVdeltaG. See https://www.ncbi.nlm.nih.gov/pubmed/19057867 for original paper on VSV pseudotyping, and https://www.cell.com/cell/pdf/S0092-8674(20)30229-4.pdf on more recent efforts with SARS-CoV-2.
Please note: Addgene NGS finds that the AmpR resistance marker is in the opposite orientation in the plasmid relative to the depositor's sequence. As the plasmid was able to grow in media with Ampicillin, this is not expected to affect plasmid function.
Proper citation: RRID:Addgene_149543 Copy
Species: Rattus norvegicus
Genetic Insert: Glp1-R
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1326760
Proper citation: RRID:Addgene_14944 Copy
Species: Synthetic
Genetic Insert: ERT2-Cre-ERT2 fusion protein
Vector Backbone Description: Backbone Marker:Liqun Luo Lab (Addgene Plasmid #40026); Backbone Size:14483; Vector Backbone:pROSA26-TG; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33082936
Proper citation: RRID:Addgene_149436 Copy
Species: Homo sapiens
Genetic Insert: Fcgamma RIIa
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17110435
Comments: The FcgRIIa cDNA was amplified by RT-PCR using a Titan One Tube RT-PCR System kit (Roche, Mannheim, Germany) and a pair of primers (5'-TTGAATTCACCATGGAGACCCAAATGTCTC-3' and 5'-GCGGCCGCTTAGTTATTACTGTTGACATGG-3'). The PCR product was cloned into pCR2.1-TOPO (Invitrogen, Carlsbad, CA) to generate pAC01 and subcloned as an EcoRI–NotI fragment into pIRESneo2 (Clontech, Mountain View, CA) to generate pCMV-FcgRIIa-IRES-Neo.
Proper citation: RRID:Addgene_14948 Copy
Vector Backbone Description: Backbone Size:4493; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.77, Ligation number NONE.
Proper citation: RRID:Addgene_1495 Copy
Species: Homo sapiens
Genetic Insert: Rheb1
Vector Backbone Description: Backbone Size:5300; Vector Backbone:HA-GST-pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17386266
Proper citation: RRID:Addgene_14951 Copy
Species: Homo sapiens
Genetic Insert: rap2a
Vector Backbone Description: Backbone Size:5300; Vector Backbone:HA-GST-pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17386266
Proper citation: RRID:Addgene_14952 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Tropix, Bedford, MA; Backbone Size:7114; Vector Backbone:pSEAP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17079315
Comments: The vector includes 5' and 3' genomic regions of the human K18 gene, necessary for driving high-level epithelial-cell-specific expression
Proper citation: RRID:Addgene_149449 Copy
Species: Synthetic
Genetic Insert: LuxR-mRFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34125913
Proper citation: RRID:Addgene_149465 Copy
Species: Homo sapiens
Genetic Insert: Smad3 (EPSM)
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10197981
Comments: 3SP and 1TP sites in the linker are mutated to alanine or valine (T-DV). See author's map for more information. Please note that the mutations correspond to residues 179, 204, 208, and 213 of the native Smad3 protein.
Proper citation: RRID:Addgene_14963 Copy
Genetic Insert: iSpyMacCas9
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible ziSpyMacCas9 entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149553 Copy
Genetic Insert: hAID-ziSpyMacCas9(D10A)-UGI
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149554 Copy
Genetic Insert: ziSpyMacCas9(D10A)-PmCDA1-UGI
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898973
Proper citation: RRID:Addgene_149555 Copy
Species: Homo sapiens
Genetic Insert: Smad1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8637600
Comments: See author's map for more information.
Proper citation: RRID:Addgene_14956 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16172383
Comments: Site directed mutagenesis in the coding sequence of Tyr162 (from TAT to TAC) and Val163 (from GTG to GTT). The mutated sequence encodes the same amino acids. See author's map for more information.
Proper citation: RRID:Addgene_14959 Copy
Species: RNase H1 (H. sapiens), dCas9 (S. pyogenes)
Genetic Insert: RNase H1-EGFP-dCas9
Vector Backbone Description: Backbone Marker:Life Technologies/Thermo Fisher; Backbone Size:5078; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32669707
Proper citation: RRID:Addgene_139835 Copy
Species: Mus musculus
Genetic Insert: Stat5
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30530727
Proper citation: RRID:Addgene_139832 Copy
Species: Mus musculus
Genetic Insert: Pu.1
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30530727
Proper citation: RRID:Addgene_139839 Copy
Species: Mus musculus
Genetic Insert: Batf3
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30530727
Proper citation: RRID:Addgene_139837 Copy
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