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Vector Backbone Description: Backbone Size:4800; Vector Backbone:Clover-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22961245
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 505; Emission = 515
Proper citation: RRID:Addgene_54575 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mTagBFP2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22174863
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 399; Emission = 456
Proper citation: RRID:Addgene_54572 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mT-Sapphire-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18425137
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 399; Emission = 511
Proper citation: RRID:Addgene_54571 Copy
Vector Backbone Description: Backbone Marker:Phil Soriano, Addgene Plasmid #21714; Vector Backbone:ROSA26-1; Vector Types:Mammalian Expression, Mouse Targeting, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24166816
Comments: Unique HpaI and XhoI sites were maintained for single step short hairpin oligonucleotide cloning.
Using the primers described above yields 231bp and 177bp for positive and negative products, respectively.
Proper citation: RRID:Addgene_54479 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mCerulean-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21479270
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_54666 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mRFP1-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12060735
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 584; Emission = 607
Proper citation: RRID:Addgene_54667 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mEGFP-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 488; Emission = 507
Proper citation: RRID:Addgene_54622 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mCherry-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15558047
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_54630 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mRuby-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 558; Emission = 605.
Addgene sequencing finds 1 mismatch to depositor's sequence(G211C), which causes L31F aa change. This is consistent with other isoforms of mRuby.
Proper citation: RRID:Addgene_54763 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mCardinal-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633408
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 604; Emission = 659
Proper citation: RRID:Addgene_54800 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:Emerald-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 487; Emission = 509
Proper citation: RRID:Addgene_54776 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mRuby2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22961245
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 559; Emission = 600
Proper citation: RRID:Addgene_54771 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mTopaz-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 514; Emission = 527
Proper citation: RRID:Addgene_54841 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mVenus-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11753368
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 515; Emission = 528
Proper citation: RRID:Addgene_54845 Copy
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mTurquoise2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_54844 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:rsEmerald-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 491; Emission = 508
Proper citation: RRID:Addgene_54850 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mOrange-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 548; Emission = 562
Proper citation: RRID:Addgene_54751 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:TurboRFP-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17572680
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 553; Emission = 574
Proper citation: RRID:Addgene_54858 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:tdTurboRFP-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17572680
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 553; Emission = 576
Proper citation: RRID:Addgene_54857 Copy
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system.
The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z).
2RRT has a TEV-cleavable N-terminal StrepII-StrepII tag.
To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers.
Forward - 5'-TACTTCCAATCCAATGCA-3'
Reverse - 5'-TTATCCACTTCCAATGTTATTA-3'
Linearize the plasmid with SspI and gel purify.
When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector.
Proper citation: RRID:Addgene_55215 Copy
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