Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Synthetic
Genetic Insert: human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene Plasmid 43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139987 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139991 Copy
Species: Synthetic
Genetic Insert: human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
Vector Backbone Description: Backbone Marker:Ketih Joung; Vector Backbone:pRZ33 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139999 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139997 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
Vector Backbone Description: Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Comments: The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids.
Proper citation: RRID:Addgene_140002 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
Vector Backbone Description: Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Comments: The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids.
Proper citation: RRID:Addgene_140003 Copy
Species: Synthetic
Genetic Insert: human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
Vector Backbone Description: Backbone Marker:Ketih Joung; Vector Backbone:pRZ33; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_140000 Copy
Species: Mus musculus
Genetic Insert: Bmp4
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRIAS; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: From Devyn Smith.
Proper citation: RRID:Addgene_14001 Copy
Species: Drosophila melanogaster
Genetic Insert: RhoA
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11239392
Proper citation: RRID:Addgene_14002 Copy
Species: Synthetic
Genetic Insert: pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Vector Backbone Description: Backbone Marker:Joung Lab (DR274); Vector Backbone:DR274 (Addgene Plasmid #42250); Vector Types:Bacterial Expression, In vitro transcription (mRNA synthesis); Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_140082 Copy
Species: Aequorea victoria
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30260624
Proper citation: RRID:Addgene_140015 Copy
Species: Methanomethylophilus alvus Mx1201
Genetic Insert: Mx1201 PylRS
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30260624
Comments: Please note there is a T72A point mutation in Neo/KanR. Depositor confirms this does not affect function.
Proper citation: RRID:Addgene_140011 Copy
Species: Escherichia coli
Genetic Insert: EcoTyrRS
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37935196
Proper citation: RRID:Addgene_140018 Copy
Species: Synthetic
Genetic Insert: Salsa6f
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3923; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29239725
Comments: The fusion protein in this plasmid is the fluorescent genetically encoded calcium indicator Salsa6f, which is a fusion of the dimeric red fluorescent protein tdTomato, the epitope tag V5, and the green fluorescent genetically encoded calcium indicator GCaMP6f, in order from N- to C-termini. The presence of tdTomato enables visualization of transfected cells in the absence of Ca2+ signaling and concentration-independent, ratiometric readout of Ca2+ signals. Salsa6f has been introduced into transgenic mice, where its expression is under Cre recombinase control (see PMID: 29239725).
Proper citation: RRID:Addgene_140188 Copy
Species: Other
Genetic Insert: FlaviA-GFP
Vector Backbone Description: Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-FlaviA-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140088 Copy
Species: Mus musculus
Genetic Insert: Tcf4B
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14667415
Proper citation: RRID:Addgene_14019 Copy
Species: Synthetic
Genetic Insert: sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
Vector Backbone Description: Vector Backbone:pUCIDT; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336
Proper citation: RRID:Addgene_140096 Copy
Species: Gallus gallus
Genetic Insert: Slug
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Slax13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full length coding sequence, PCR (vent) product, 5’ primer converts 2nd codon from cca (pro) to gca (ala) in order to generate a Nco I site; 3’ primer is wt plus EcoRI site, from Malcolm Logan.
Proper citation: RRID:Addgene_14015 Copy
Species: Other
Genetic Insert: FlaviA-mNeptune
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Backbone Size:7871; Vector Backbone:pLenti CMV GFP Puro (658-5) (Addgene Plasmid #17448); Vector Types:Mammalian Expression, Lentiviral, High expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-mNeptune reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter and/or generate OSER structures - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-CMV-FlaviA-mNeptune-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140091 Copy
Species: Homo sapiens
Genetic Insert: Src mutant
Vector Backbone Description: Backbone Marker:modified from ThermoFisher 12274015; Backbone Size:7100; Vector Backbone:modified pcDNA-pDEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30304684
Proper citation: RRID:Addgene_140312 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
