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Species: Mus musculus
Genetic Insert: Frat2
Vector Backbone Description: Backbone Marker:Olivier Destree; Backbone Size:5500; Vector Backbone:pGlomyc (based on pCDNA3.1); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31961879
Comments: References:
https://www.ncbi.nlm.nih.gov/pubmed/?term=10534617
https://www.ncbi.nlm.nih.gov/pubmed/?term=15073180
https://www.ncbi.nlm.nih.gov/pubmed/?term=19802005 Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124500 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31961879
Comments: Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124502 Copy
Species: Mus musculus
Genetic Insert: UBP43
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4500; Vector Backbone:pBK-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10082570
Proper citation: RRID:Addgene_12459 Copy
Species: Mus musculus
Genetic Insert: Soma-localized C1V1(t/t)
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:3000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842660
Proper citation: RRID:Addgene_124604 Copy
Species: Mus musculus
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30295801
Proper citation: RRID:Addgene_124550 Copy
Species: Mus musculus
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30295801
Proper citation: RRID:Addgene_124552 Copy
Species: Mus musculus
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30295801
Proper citation: RRID:Addgene_124551 Copy
Species: Mus musculus
Genetic Insert: ChrimsonR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842660
Proper citation: RRID:Addgene_124651 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAC-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31961879
Comments: The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector).
To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Please note: The plasmid contains P13S, H81Y, and I229T mutations in SGFP2. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_124503 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAC-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31961879
Comments: The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector).
To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted.
The mCherry tagged variant was generated by exchanging an AgeI/BsrgI sGFP2 fragment for an AgeI/BsrgI mTurquoise fragment. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124505 Copy
Species: Mus musculus
Genetic Insert: Myo1e
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30601698
Proper citation: RRID:Addgene_124658 Copy
Species: Mus musculus
Genetic Insert: Murine IgG3
Vector Backbone Description: Backbone Size:4706; Vector Backbone:pHybr_r2a; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124805 Copy
Species: Mus musculus
Genetic Insert: Murine IgG2a
Vector Backbone Description: Backbone Size:4706; Vector Backbone:pHybr_r2a; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124803 Copy
Species: Mus musculus
Genetic Insert: Murine IgG2a (Fc silent)
Vector Backbone Description: Backbone Size:4706; Vector Backbone:pHybr_r2a; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124807 Copy
Species: Mus musculus
Genetic Insert: Murine IgG1
Vector Backbone Description: Backbone Size:4706; Vector Backbone:pHybr_r2a; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124802 Copy
Species: Mus musculus
Genetic Insert: Gabrg3
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124858 Copy
Species: Mus musculus
Genetic Insert: Slc17a7
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124859 Copy
Species: Mus musculus
Genetic Insert: Gria1
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124853 Copy
Species: Mus musculus
Genetic Insert: Grin2a
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124850 Copy
Species: Mus musculus
Genetic Insert: Gabrg1
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124856 Copy
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