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Species: Mus musculus
Genetic Insert: Sema3e
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72146 Copy
Species: Mus musculus
Genetic Insert: Sema3f
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72147 Copy
Species: Mus musculus
Genetic Insert: Sema6d.x
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Sema6d.x is missing exons 16b and 17.
Proper citation: RRID:Addgene_72173 Copy
Species: Mus musculus
Genetic Insert: Unc5a.z
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Unc5a.z is missing exon 6.
Proper citation: RRID:Addgene_72177 Copy
Species: Mus musculus
Genetic Insert: Unc5b.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: We identified a previously-unreported isoform and named it "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72178 Copy
Species: Mus musculus
Genetic Insert: Unc5a.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: We identified a previously-unreported isoform and named it "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72176 Copy
Species: Mus musculus
Genetic Insert: Sema6a.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: We identified a previously-unreported isoform and named it "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72163 Copy
Species: Mus musculus
Genetic Insert: Sema4g
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72160 Copy
Species: Mus musculus
Genetic Insert: Sema6b
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Addgene Sanger sequencing found F187L within the Sema6b translation compared to NM_013662.2.
Proper citation: RRID:Addgene_72166 Copy
Species: Mus musculus
Genetic Insert: Sema6c
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72167 Copy
Species: Mus musculus
Genetic Insert: Sema6d.1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72168 Copy
Species: Mus musculus
Genetic Insert: Unc5c
Vector Backbone Description: Backbone Marker:Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72196 Copy
Species: Mus musculus
Genetic Insert: Unc5a
Vector Backbone Description: Backbone Marker:Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72194 Copy
Species: Mus musculus
Genetic Insert: Unc5d
Vector Backbone Description: Backbone Marker:Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72197 Copy
Species: Mus musculus
Genetic Insert: Unc5c.y
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Unc5c.y has a 57 nt insertion between exons 7 and 8.
Proper citation: RRID:Addgene_72181 Copy
Species: Mus musculus
Genetic Insert: Unc5d.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: We identified two previously-unreported isoforms and named them "y" and "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72183 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 3
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
Note that an additional mutation, D1507E, is present in the Tet3 insert in this plasmid. This additional mutation does not affect the property of the mutant protein (as it is catalytically dead).
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72222 Copy
Species: Mus musculus
Genetic Insert: MEK1/2
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26942229
Proper citation: RRID:Addgene_72570 Copy
Species: Mus musculus
Genetic Insert: MEK1/2
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26942229
Proper citation: RRID:Addgene_72568 Copy
Species: Mus musculus
Genetic Insert: mouse TNF-alpha shRNA
Vector Backbone Description: Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24642694
Proper citation: RRID:Addgene_72598 Copy
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