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Species: Homo sapiens
Genetic Insert: lin-28 homolog B
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe.puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19483683
Proper citation: RRID:Addgene_26358 Copy
Species: Homo sapiens
Genetic Insert: Sox2 shRNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801978
Comments: SOX2a shRNA sequence - CGAGATAAACATGGCAATCAA
Proper citation: RRID:Addgene_26353 Copy
Species: Homo sapiens
Genetic Insert: Sox2 shRNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801978
Comments: SOX2b shRNA sequence - CTGCCGAGAATCCATGTATAT
Proper citation: RRID:Addgene_26352 Copy
Species: Homo sapiens
Genetic Insert: Sox2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801978
Proper citation: RRID:Addgene_26351 Copy
Species: Homo sapiens
Genetic Insert: FOXE1
Vector Backbone Description: Backbone Marker:Addgene Plasmid 1764; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801978
Proper citation: RRID:Addgene_26350 Copy
Species: Homo sapiens
Genetic Insert: miR-155
Vector Backbone Description: Backbone Marker:Addgene plasmid 12282; Backbone Size:6500; Vector Backbone:pMIG-w; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18299402
Comments: MG155 was derived originally from pMIG-w. The GFP and IRES were removed using BglII and HindIII, and a new cassette with eGFP (about 700 bps) followed by a miR-155 sequence (about 500 bps with flanking Not1 and XhoI sites) was cloned in using BamHI (destroying the BglII site) and HindIII. This results in a 7 kb plasmid.
Proper citation: RRID:Addgene_26529 Copy
Species: Homo sapiens
Genetic Insert: IL7
Vector Backbone Description: Backbone Size:7707; Vector Backbone:pUC18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_26532 Copy
Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Backbone Size:6037; Vector Backbone:pIRES-puro2b; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20693996
Comments: Note that there are a few minor discrepancies between Addgene's quality control sequence and the author's sequence, but these should not affect function.
Proper citation: RRID:Addgene_26461 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26607 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26606 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26608 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26603 Copy
Species: Homo sapiens
Genetic Insert: PP1a
Vector Backbone Description: Backbone Marker:Peti Lab (Addgene #26563); Backbone Size:0; Vector Backbone:RP1B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18992256
Comments: The backbone is a modified version of pET28 and is also available at Addgene (plasmid #26563)
Proper citation: RRID:Addgene_26566 Copy
Species: Homo sapiens
Genetic Insert: Apoptotic protease activating factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630894
Proper citation: RRID:Addgene_26642 Copy
Species: Homo sapiens
Genetic Insert: Rheb1 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: shRNA directed against Rheb1. Target sequence: 5'-CCTCAGACATACTCCATAGAT-3'.
Proper citation: RRID:Addgene_26625 Copy
Species: Homo sapiens
Genetic Insert: RagB shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: shRNA directed against RagB. Target sequence: 5'-CGGGACAACATCTTCCGAAAT-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Proper citation: RRID:Addgene_26627 Copy
Species: Homo sapiens
Genetic Insert: Rheb1 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: shRNA directed against Rheb1. Target sequence: 5'-TTATGTTGGTTGGGAATAAGA-3'.
Proper citation: RRID:Addgene_26626 Copy
Species: Homo sapiens
Genetic Insert: p18 shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20381137
Comments: shRNA directed against p18. Target sequence: 5'-AGACAGCCAGCAACATCATTG-3'
Proper citation: RRID:Addgene_26631 Copy
Species: Homo sapiens
Genetic Insert: raptor
Vector Backbone Description: Backbone Marker:Available at Addgene (#19319); Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20381137
Proper citation: RRID:Addgene_26633 Copy
Species: Homo sapiens
Genetic Insert: STAT3 shRNA
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7200; Vector Backbone:pSIH1-H1-puro; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20197401
Comments: STAT3 shRNA sequence: gatccGCATCTGCCTAGATCGGCTATTCAAGAGATAGCCGATCTAGGCAGATGTTTTTTg
Proper citation: RRID:Addgene_26596 Copy
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